ENO1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

ENO1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1D6E8

Kat-Nr. : 67187-1-Ig

Synonyme

1D6E8, 2-phospho-D-glycerate hydro-lyase, Alpha-enolase, C-myc promoter-binding protein, EC:4.2.1.11

---

• 

  WB analysis of HEK-293 using 67187-1-Ig

  WB result of ENO1 antibody (67187-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1 transfected HEK-293 cells.

• 

  WB analysis using 67187-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67187-1-Ig (ENO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 67187-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ENO1 antibody (67187-1-Ig, Clone: 1D6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of HeLa using 67187-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ENO1 antibody (67187-1-Ig, Clone: 1D6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, PC-3-Zellen, Hausschwein-Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67187-1-Ig bindet in WB, IF/ICC, ELISA ENO1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ENO1 fusion protein Ag17518
• Vollständiger Name: enolase 1, (alpha)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC015641
• Gene symbol: ENO1
• Gene ID (NCBI): 2023
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Thiophiler Affinitätschromatograph
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656)

Publikationen

Species	Application	Title
human	WB	Tissue Cell; Activation of mTOR/HK2 signaling mitigates effects of PYCR2 depletion in colorectal cells; Authors - Li Chen