NSE Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

NSE Monoklonal Antikörper für WB, IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(27)

CloneNo.

6F8G3

Rezensionen (1)

Kat-Nr. : 66150-1-Ig

Synonyme

ENO2, 2-phospho-D-glycerate hydro-lyase, 6F8G3, EC:4.2.1.11, Enolase 2

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  WB analysis using 66150-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of U-251 using 66150-1-Ig

  WB result of NSE antibody (66150-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE transfected U-251 cells.

• 

  WB analysis of Neuro-2a using 66150-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 66150-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 66150-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66150-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66150-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66150-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 66150-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 66150-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66150-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 66150-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66150-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66150-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66150-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66150-1-Ig (NSE antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 66150-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66150-1-Ig (NSE antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat cerebellum using 66150-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 66150-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66150-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66150-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 66150-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSE antibody (66150-1-Ig, Clone: 6F8G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 66150-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSE antibody (66150-1-Ig, Clone: 6F8G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of SH-SY5Y using 66150-1-Ig

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NSE (66150-1-Ig, Clone:6F8G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, C6-Zellen, fetales humanes Hirngewebe, HEK-293-Zellen, Maushirngewebe, Maus-Cerebellum-Gewebe, Neuro-2a-Zellen, Hausschwein-Hirngewebe, Hausschwein-Cerebellum-Gewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe, SH-SY5Y-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 10 publications below

Produktinformation

66150-1-Ig bindet in WB, IHC, IF-P, FC (Intra), ELISA NSE und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NSE fusion protein Ag19106
• Vollständiger Name: enolase 2 (gamma, neuronal)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC002745
• Gene symbol: NSE
• Gene ID (NCBI): 2026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
human	IF	Oncogenesis; Neuron-specific enolase promotes stem cell-like characteristics of small-cell lung cancer by downregulating NBL1 and activating the BMP2/Smad/ID1 pathway.; Authors - Lin Lu
rat	IHC	J Neurochem; Extracellular ATP and cAMP signaling promote Piezo2-dependent mechanical allodynia after trigeminal nerve compression injury.; Authors - Zhaoke Luo
rat	IF	CNS Neurosci Ther; Sema7A, a brain immune regulator, regulates seizure activity in PTZ-kindled epileptic rats.; Authors - Jing Deng
Rat		PLoS One; Supplementation with dietary omega-3 PUFA mitigates fetal brain inflammation and mitochondrial damage caused by high doses of sodium nitrite in maternal rats.; Authors - Jingchi Sun
rat	IHC	Neuroreport; Effects of morphine preconditioning on TRPV1 sensitization and ERK1/2 phosphorylation induced by TGFβ1 in neurocytes.; Authors - Zhen-Xiao Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Morgan (Verified Customer) (10-26-2021)

Overall it worked well. It looked a little dirty with some "extra" bands, but I'm not exactly sure why. The trend is still clear though.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: OV90 Ovarian Cancer Cells

NSE Antibody Western Blot validation (1:500 dilution) in OV90 Ovarian Cancer Cells (Cat no:66150-1-Ig)