ENOPH1 Polyklonaler Antikörper

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• KD/KO Validated

ENOPH1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 11763-1-AP

Synonyme

E1, enolase phosphatase 1, Enolase phosphatase E1, ENOPH1, MASA, MASA homolog, MST145

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  WB analysis using 11763-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11763-1-AP (ENOPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 11763-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11763-1-AP (ENOPH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HL-60-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

11763-1-AP bindet in WB, IF, ELISA ENOPH1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ENOPH1 fusion protein Ag2360
• Vollständiger Name: enolase-phosphatase 1
• Berechnetes Molekulargewicht: 13 kDa / 29 kDa
• Beobachtetes Molekulargewicht: 29-35 kDa
• GenBank-Zugangsnummer: BC001317
• Gene symbol: ENOPH1
• Gene ID (NCBI): 58478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENOPH1, also named as MASA and MSTP145, belongs to the HAD-like hydrolase superfamily and MasA/MtnC family. ENOPH1 is a newly identified enzyme involved in L-methionine biosynthesis, which is associated with anxiety and depression. Studies have shown that ENOPH1 plays a crucial role in promoting the proliferation and migration of glioma cells (PMID: 32654229). ENOPH1 has 2 isoforms with the molecular mass of 13 and 29 kDa.

Publikationen

Species	Application	Title
human	WB,IF	Front Mol Neurosci; Cerebral Microvascular Endothelial Cell Apoptosis after Ischemia: Role of Enolase-Phosphatase 1 Activation and Aci-Reductone Dioxygenase 1 Translocation.; Authors - Yuan Zhang ; KD Validated
human	WB	Oncol Rep; Enolase-phosphatase 1 as a novel potential malignant glioma indicator promotes cell proliferation and migration.; Authors - Li Su ; KD Validated