ENOSF1 Rekombinanter Antikörper

ENOSF1 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240796D2

Kat-Nr. : 83769-2-RR

Synonyme

TYMSAS, rTS, L-fuconate dehydratase, enolase superfamily member 1, EC:4.2.1.68

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  WB analysis using 83769-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83769-2-RR (ENOSF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of Hela using 83769-2-RR

  1x10^6 Hela cells were intracellularly stained with 0.25 ug ENOSF1 Recombinant antibody (83769-2-RR, Clone:240796D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83769-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83769-2-RR against Human ENOSF1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83769-2-RR bindet in WB, FC (Intra), ELISA ENOSF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ENOSF1 fusion protein Ag34665
• Vollständiger Name: enolase superfamily member 1
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC001285
• Gene symbol: ENOSF1
• Gene ID (NCBI): 55556
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENOSF1 (Mitochondrial enolase superfamily member 1) is also named as RTS and TYMSAS. The ENOSF1 gene, responsible for encoding the protein mitochondrial enolase superfamily member 1 (ENOF1), exhibits three distinct isoforms. One of these isoforms has been identified as an l-fuconate dehydratase, actively participating in the catabolism of L-fucose, a sugar integral to the carbohydrate composition of cellular glycoproteins (PMID: 38203276). The ENOSF1 transcript has been shown to act as antisense RNA and inhibit TYMS expression (PMID: 35931051).