ENOX2 Polyklonaler Antikörper

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• KD/KO Validated

ENOX2 Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-10423

Synonyme

Protein disulfide-thiol oxidoreductase, Hydroquinone [NADH] oxidase, Ecto-NOX disulfide-thiol exchanger 2, COVA1, APK1 antigen

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  IF Staining of MCF-7 using CL488-10423

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 ENOX2 antibody (CL488-10423) at dilution of 1:200.

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  FC experiment of MCF-7 using CL488-10423

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated ENOX2 Polyclonal antibody (CL488-10423)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-10423 bindet in IF/ICC, FC (Intra) ENOX2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ENOX2 fusion protein Ag0674
• Vollständiger Name: ecto-NOX disulfide-thiol exchanger 2
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC019254
• Gene symbol: ENOX2
• Gene ID (NCBI): 10495
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor-associated NADH oxidase (ENOX2) as a member of a family of growth-related NADH (or hydroquinone) oxidases. It functions as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock