ENPP2 Polyklonaler Antikörper

ENPP2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Rezensionen (1)

Kat-Nr. : 14243-1-AP

Synonyme

ATX, ATX X, Autotaxin, E NPP 2, ENPP2, LysoPLD, PD IALPHA, PDNP2

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  WB analysis using 14243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14243-1-AP (Autotaxin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14243-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14243-1-AP (Autotaxin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14243-1-AP

  IP result of anti-Autotaxin (IP:14243-1-AP, 5ug; Detection:14243-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human small intestine using 14243-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14243-1-AP (Autotaxin antibody at dilution of 1:50.

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  IHC staining of human liver cancer using 14243-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14243-1-AP (Autotaxin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 14243-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14243-1-AP (Autotaxin antibody at dilution of 1:50.

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  IHC staining of human liver cancer using 14243-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14243-1-AP (Autotaxin antibody at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Plazenta-Gewebe, Mausnierengewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 3 publications below

Produktinformation

14243-1-AP bindet in WB, IHC, IP, ELISA ENPP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ENPP2 fusion protein Ag5499
• Vollständiger Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
• Berechnetes Molekulargewicht: 99 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC034961
• Gene symbol: ENPP2
• Gene ID (NCBI): 5168
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENPP2, Autotaxin or Extracellular lysophospholipase D, is a 863 amino acid secreted protein, which is detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). ENPP2 is Predominantly expressed in brain, placenta, ovary, and small intestine. ENPP2 is expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. ENPP2 is expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. The calculated molecular weight of ENPP2 is about 100 kDa (PMID: 22301782), but modified ENPP2 is 120 kDa (PMID: 19808661). ENPP2 Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931).

Publikationen

Species	Application	Title
mouse	WB	Mol Psychiatry; Transthyretin, a novel prognostic marker of POCD revealed by time-series RNA-sequencing analysis; Authors - Xiaosheng Liang
human	WB	PLoS Pathog; Reduced IRF4 expression promotes lytic phenotype in Type 2 EBV-infected B cells.; Authors - Jillian A Bristol
human,mouse	WB	Cells; Role of Aberrantly Activated Lysophosphatidic Acid Receptor 1 Signaling Mediated Inflammation in Renal Aging.; Authors - Yongjie Jin
mouse	WB	Front Pharmacol; UPLC/Q-TOFMS-Based Metabolomics Approach to Reveal the Protective Role of Other Herbs in An-Gong-Niu-Huang Wan Against the Hepatorenal Toxicity of Cinnabar and Realgar.; Authors - Fangbo Xia
human	IHC	Heliyon; Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study; Authors - Lingyan Chen
mouse	WB	Am J Physiol Cell Physiol; The Ecto-Nucleotide Pyrophosphatase/Phosphodiesterase 2 Promotes Early Osteoblast Differentiation and Mineralization in Stromal Stem Cells; Authors - Irina L Tourkova

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jacqueline (Verified Customer) (05-14-2019)

Works perfectly in western blot using concentrated media of cells that are cultured in serum-depleted conditions. Serum is a good positive control or secreted ENPP2/ATX. In our hands ~120kD

• Applications: Western Blot, Immunoprecipitation, Cell culture,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Media from PDAC cells and fibroblasts

ENPP2 Antibody Western Blot,Immunoprecipitation,Cell culture, validation (1:500 dilution) in Media from PDAC cells and fibroblasts (Cat no:14243-1-AP)