ENT1 Polyklonaler Antikörper

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ENT1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 29862-1-AP

Synonyme

ENT-1, Equilibrative NBMPR-sensitive nucleoside transporter, Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, es nucleoside transporter, Nucleoside transporter, es-type

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  WB analysis using 29862-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29862-1-AP (ENT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 29862-1-AP

  WB result of ENT1 antibody (29862-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENT1 transfected HeLa cells.

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  WB analysis using 29862-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29862-1-AP (ENT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 29862-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29862-1-AP (ENT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 29862-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 29862-1-AP (ENT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HeLa-Zellen, Mausherzgewebe, Mausnierengewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

29862-1-AP bindet in WB, IHC, IF, ELISA ENT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ENT1 fusion protein Ag32003
• Vollständiger Name: solute carrier family 29 (nucleoside transporters), member 1
• Berechnetes Molekulargewicht: 50KD
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: NM_001372327.1
• Gene symbol: ENT1
• Gene ID (NCBI): 2030
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENT1 (equilibrative nucleoside transporter 1; also known as SLC29A1) is a ubiquitous protein located at the cell membrane and is a major transporter responsible for the cellular uptake and release of endogenous nucleosides such as adenosine, and nucleoside analogs used in chemotherapy. The predicted molecular weight of ENT1 is 50 kDa, while heavier 54-68 kDa band may be observed due to the glycosylation (PMID: 16448802, 11850433, 16111480). A smaller novel splice variant of the mouse ENT1 has also been identified, which generates a protein of 35-40 kDa (PMID: 18413666).

Publikationen

Species	Application	Title
human	WB,IF	Fluids Barriers CNS; Cocaine regulates antiretroviral therapy CNS access through pregnane-x receptor-mediated drug transporter and metabolizing enzyme modulation at the blood brain barrier; Authors - Rodnie Colón Ortiz
mouse	WB	Hippocampus; Anticonvulsant effect of equilibrative nucleoside transporters 1 inhibitor in a mouse model of Dravet syndrome; Authors - Shih-Yin Ho
	IF	Proc Natl Acad Sci U S A; Pharmacological inhibition of ENT1 enhances the impact of specific dietary fats on energy metabolism gene expression; Authors - Erwann Pain