CD39/ENTPD1 Polyklonaler Antikörper
CD39/ENTPD1 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF-P, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 19229-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Mauslebergewebe, HL-60-Zellen, humanes Lebergewebe, Maushirngewebe |
| Erfolgreiche IP | Mauslebergewebe |
| Erfolgreiche Detektion in IHC | humanes Plazenta-Gewebe, humanes Lebergewebe, humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF-P | humanes Plazenta-Gewebe |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:20-1:200 |
| Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Produktinformation
19229-1-AP bindet in WB, IHC, IF-P, IP, ELISA CD39/ENTPD1 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | CD39/ENTPD1 fusion protein Ag6165 |
| Vollständiger Name | ectonucleoside triphosphate diphosphohydrolase 1 |
| Berechnetes Molekulargewicht | 58 kDa |
| Beobachtetes Molekulargewicht | 75-95 kDa |
| GenBank-Zugangsnummer | BC047664 |
| Gene symbol | CD39 |
| Gene ID (NCBI) | 953 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). It has 3 isoforms produced by alternative splicing. CD39 preferentially occurred as a dimer that could be dissociated into monomeric forms. (PMID: 12694193). The soluble isoform of CD39(38-478 amino acid) is located in the extracellular part and 19229-1-AP recognizes both the 3 isoforms and the soluble form of CD39 (38-478aa) molecule.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for CD39/ENTPD1 antibody 19229-1-AP | Protokoll herunterladen |
| IHC protocol for CD39/ENTPD1 antibody 19229-1-AP | Protokoll herunterladenl |
| IF protocol for CD39/ENTPD1 antibody 19229-1-AP | Protokoll herunterladen |
| IP protocol for CD39/ENTPD1 antibody 19229-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Bone Res Alpl prevents bone ageing sensitivity by specifically regulating senescence and differentiation in mesenchymal stem cells. | ||
Biochim Biophys Acta Mol Basis Dis Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages | ||
J Cell Mol Med CD39 Contributes to the Ability of Cell Invasion in Heterogeneity of Colorectal Cancer | ||
J Chem Inf Model Identification of Novel CD39 Inhibitors Based on Virtual Screening and Enzymatic Assays. | ||
Biochem J Characterization of soluble CD39 (SolCD39/NTPDase1) from PiggyBac nonviral system as a tool to control the nucleotides level. | ||
JCI Insight Epithelial microRNA-206 targets CD39/extracellular ATP to upregulate airway IL-25 and TSLP in type 2-high asthma. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Isabelle (Verified Customer) (10-08-2020) | good antibody for western blot
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