CD39/ENTPD1 Polyklonaler Antikörper

CD39/ENTPD1 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 19229-1-AP

Synonyme

ENTPD1, ATP diphosphohydrolase, ATPDase, ATP-DPH, CD39



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMauslebergewebe, HL-60-Zellen, humanes Lebergewebe, Maushirngewebe
Erfolgreiche IPMauslebergewebe
Erfolgreiche Detektion in IHChumanes Plazenta-Gewebe, humanes Lebergewebe, humanes Tonsillitisgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-Phumanes Plazenta-Gewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

19229-1-AP bindet in WB, IHC, IF-P, IP, ELISA CD39/ENTPD1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD39/ENTPD1 fusion protein Ag6165
Vollständiger Name ectonucleoside triphosphate diphosphohydrolase 1
Berechnetes Molekulargewicht 58 kDa
Beobachtetes Molekulargewicht 75-95 kDa
GenBank-ZugangsnummerBC047664
Gene symbol CD39
Gene ID (NCBI) 953
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). It has 3 isoforms produced by alternative splicing. CD39 preferentially occurred as a dimer that could be dissociated into monomeric forms. (PMID: 12694193). The soluble isoform of CD39(38-478 amino acid) is located in the extracellular part and 19229-1-AP recognizes both the 3 isoforms and the soluble form of CD39 (38-478aa) molecule.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CD39/ENTPD1 antibody 19229-1-APProtokoll herunterladen
IHC protocol for CD39/ENTPD1 antibody 19229-1-APProtokoll herunterladenl
IF protocol for CD39/ENTPD1 antibody 19229-1-APProtokoll herunterladen
IP protocol for CD39/ENTPD1 antibody 19229-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Bone Res

Alpl prevents bone ageing sensitivity by specifically regulating senescence and differentiation in mesenchymal stem cells.

Authors - Wenjia Liu
humanWB

Biochim Biophys Acta Mol Basis Dis

Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages

Authors - Ling Zhou
humanIF

J Cell Mol Med

CD39 Contributes to the Ability of Cell Invasion in Heterogeneity of Colorectal Cancer

Authors - Xiaosong Li
human

J Chem Inf Model

Identification of Novel CD39 Inhibitors Based on Virtual Screening and Enzymatic Assays.

Authors - Yunshuo Zhao
ratWB

Biochem J

Characterization of soluble CD39 (SolCD39/NTPDase1) from PiggyBac nonviral system as a tool to control the nucleotides level.

Authors - Liziane Raquel Beckenkamp
mouseIHC

JCI Insight

Epithelial microRNA-206 targets CD39/extracellular ATP to upregulate airway IL-25 and TSLP in type 2-high asthma.

Authors - Kan Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Isabelle (Verified Customer) (10-08-2020)

good antibody for western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: human liver, hepG2 cell line, renal tubular HK2 cell line