CD39/ENTPD1 Polyklonaler Antikörper

CD39/ENTPD1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 19229-1-AP

Synonyme

ATPDase, CD39, CD39/ENTPD1, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, e-NTPDase, NTPDase 1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 19229-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 19229-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 19229-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19229-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19229-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19229-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19229-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 19229-1-AP

  IP Result of anti-CD39 (IP:19229-1-AP, 4ug; Detection:19229-1-AP 1:800) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human placenta using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 19229-1-AP (CD39 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human tonsillitis using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 19229-1-AP (CD39 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of human placenta using 19229-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD39/ENTPD1 antibody (19229-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human placenta using 19229-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD39/ENTPD1 antibody (19229-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 19229-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug CD39 antibody (19229-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HL-60-Zellen, humanes Lebergewebe, Maushirngewebe, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Lebergewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

19229-1-AP bindet in WB, IP, IHC, IF, FC, ELISA CD39/ENTPD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD39/ENTPD1 fusion protein Ag6165
• Vollständiger Name: ectonucleoside triphosphate diphosphohydrolase 1
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 75-95 kDa
• GenBank-Zugangsnummer: BC047664
• Gene symbol: ENTPD1
• Gene ID (NCBI): 953
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named as CD39, NTPDase 1, Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.It is a noncovalent tetrameric protein and that detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ).And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). It has 3 isoforms produced by alternative splicing. CD39 preferentially occurred as a dimer that could be dissociated into monomeric forms. (PMID: 12694193). The soluble isoform of CD39(38-478 amino acid) locates to the extracellular part and 19229-1-AP recognizes both the 3 isoforms and soluble form of CD39 (38-478aa) molecule.

Publikationen

Species	Application	Title
mouse	WB	Bone Res; Alpl prevents bone ageing sensitivity by specifically regulating senescence and differentiation in mesenchymal stem cells.; Authors - Wenjia Liu
human	WB	Biochim Biophys Acta Mol Basis Dis; Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages; Authors - Ling Zhou
human	FC	J Chem Inf Model; Identification of Novel CD39 Inhibitors Based on Virtual Screening and Enzymatic Assays.; Authors - Yunshuo Zhao
rat	WB	Biochem J; Characterization of soluble CD39 (SolCD39/NTPDase1) from PiggyBac nonviral system as a tool to control the nucleotides level.; Authors - Liziane Raquel Beckenkamp
mouse	IHC	JCI Insight; Epithelial microRNA-206 targets CD39/extracellular ATP to upregulate airway IL-25 and TSLP in type 2-high asthma.; Authors - Kan Zhang
mouse	WB,IHC	Cancer Immunol Immunother; STAT3 promotes differentiation of monocytes to MDSCs via CD39/CD73-adenosine signal pathway in oral squamous cell carcinoma; Authors - Hao Cui

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Isabelle (Verified Customer) (10-08-2020)

good antibody for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: human liver, hepG2 cell line, renal tubular HK2 cell line