ENTPD3 Polyklonaler Antikörper

ENTPD3 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 13021-1-AP

Synonyme

Ecto-ATPase 3, Ecto-ATP diphosphohydrolase 3, Ecto-apyrase 3, EC:3.6.1.5, CD39L3

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  WB analysis of human brain using 13021-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13021-1-AP (ENTPD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13021-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13021-1-AP (ENTPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 13021-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13021-1-AP (ENTPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 13021-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ENTPD3 antibody (13021-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Jurkat-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13021-1-AP bindet in WB, IF/ICC, ELISA ENTPD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ENTPD3 fusion protein Ag4114
• Vollständiger Name: ectonucleoside triphosphate diphosphohydrolase 3
• Berechnetes Molekulargewicht: 529 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC029869
• Gene symbol: ENTPD3
• Gene ID (NCBI): 956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENTPD3, also known as NTPDase3 or CD39L3, belongs to the GDA1/CD39 NTPase family. ENTPD3 has a threefold preference for the hydrolysis of ATP over ADP. ENTPD3 is expressed in adult brain, pancreas, spleen and prostate, but not in liver and peripheral blood leukocytes. ENTPD3 has 2 isoforms with the molecular mass of 51 and 59 kDa.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Impaired calcium signaling in astrocytes modulates autism spectrum disorder-like behaviors in mice.; Authors - Qian Wang
mouse	WB	Reprod Sci; Expression and Hormonal Regulation of Entpd3 at Various Estrous Cycle Stages in the Mouse Uterus; Authors - Dan Mo