EPCAM/CD326 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EPCAM/CD326 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

57

Kat-Nr. : 21050-1-AP

Synonyme

CD326, CO 17A, CO17 1A, EGP, EGP 2, EGP314, EGP34, EGP40, Ep CAM, EPCAM, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733 2, hEGP 2, hEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, M4S1, MIC18, MK 1, TACST 1, TACSTD1, TROP1

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Galerie der Validierungsdaten

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  WB analysis of A431 using 21050-1-AP

  WB result of EPCAM antibody (21050-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells.

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  WB analysis of A431 using 21050-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21050-1-AP (EPCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21050-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21050-1-AP (EPCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 21050-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 21050-1-AP (EPCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21050-1-AP (EPCAM antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21050-1-AP (EPCAM antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21050-1-AP (EPCAM Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21050-1-AP (EPCAM Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse stomach using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse lung using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 21050-1-AP (EPCAM antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 21050-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using EPCAM antibody (21050-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HT-29 using 21050-1-AP

  1X10^6 HT-29 cells were surface stained with 0.5 ug Anti-Human EPCAM/CD326 (21050-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were not fixed.

• 

  FC experiment of A431 using 21050-1-AP

  1X10^6 A431 cells were surface stained with 0.4 ug Anti-Human EPCAM (21050-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

• 

  FC experiment of HepG2 using 21050-1-AP

  1X10^6 HepG2 cells were surface stained with 0.4 ug Anti-Human EPCAM/CD326 (21050-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Maus-Kolongewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Kolongewebe, humanes Mammakarzinomgewebe, Maus-Kolongewebe, Maus-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Kolongewebe, Mauslungengewebe, MCF-7-Zellen
• Erfolgreiche Detektion in FC: HT-29-Zellen, A431-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 34 publications below
• IHC: See 13 publications below
• IF: See 15 publications below
• FC: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

21050-1-AP bindet in WB, IHC, IF, FC, chIP, ELISA EPCAM/CD326 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EPCAM/CD326 fusion protein Ag15393
• Vollständiger Name: epithelial cell adhesion molecule
• Berechnetes Molekulargewicht: 314 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC014785
• Gene symbol: EPCAM
• Gene ID (NCBI): 4072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

Publikationen

Species	Application	Title
human	IF	Mol Cancer; m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma; Authors - Han Fang
mouse	IHC	Cancer Cell; Loss of Lkb1 and Pten leads to lung squamous cell carcinoma with elevated PD-L1 expression.; Authors - Chunxiao Xu
mouse	IF	Gut; Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis.; Authors - Yunben Yang
human	IF	Nat Commun; Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.; Authors - Zhaohui Chen
human	WB	Biosens Bioelectron; Tumor-cell detection, labeling and phenotyping with an electron-doped bifunctional signal-amplifier.; Authors - Hao Lu
human	WB	EMBO J; A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.; Authors - Doowon Huh

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