EPCAM/CD326 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EPCAM/CD326 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, FC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, FC, IP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen(90)

Rezensionen (1)

Kat-Nr. : 21050-1-AP

Synonyme

EPCAM, EGP, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1

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  WB analysis of A431 using 21050-1-AP

  WB result of EPCAM antibody (21050-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells.

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  WB analysis using 21050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21050-1-AP (EPCAM antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21050-1-AP (EPCAM antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21050-1-AP (EPCAM Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21050-1-AP (EPCAM Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse lung using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of MCF-7 using 21050-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using EPCAM antibody (21050-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 21050-1-AP (EPCAM antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HT-29 using 21050-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of HT-29 using 21050-1-AP

  1x10^6 HT-29 cells were surface stained with 0.5 ug EPCAM/CD326 Polyclonal antibody (21050-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.5 ug Control Antibody (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Maus-Kolongewebe, Ratten-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Kolongewebe, Maus-Kolongewebe, Maus-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Kolongewebe, MCF-7-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IF-Fro: Maus-Kolongewebe
• Erfolgreiche Detektion in IF/ICC: HT-29-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC: HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:500-1:2000
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 45 publications below
• IHC: See 17 publications below
• IF: See 34 publications below
• IP: See 1 publications below
• FC: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

21050-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, FC, IP, chIP, ELISA EPCAM/CD326 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EPCAM/CD326 fusion protein Ag15393
• Vollständiger Name: epithelial cell adhesion molecule
• Berechnetes Molekulargewicht: 314 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC014785
• Gene symbol: EPCAM
• Gene ID (NCBI): 4072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

Publikationen

Species	Application	Title
human	IF	Cancer Cell; Single-cell multi-stage spatial evolutional map of esophageal carcinogenesis; Authors - Jiang Chang
human	IF	Mol Cancer; m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma; Authors - Han Fang
human,mouse	IF	Cell Metab; Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer; Authors - Yu Jiang
mouse	IHC	Cancer Cell; Loss of Lkb1 and Pten leads to lung squamous cell carcinoma with elevated PD-L1 expression.; Authors - Chunxiao Xu
mouse	IF	Gut; Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis.; Authors - Yunben Yang
	IF	Autophagy; Neutrophil-derived serine proteases induce FOXA2-mediated autophagy dysfunction and exacerbate colitis-associated carcinogenesis via F2RL1/protease-activated receptor 2; Authors - Junhu Yuan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrew (Verified Customer) (07-12-2024)

Got a clean band at the desired molecular weight for EPCAM on the first attempt of using this primary antibody for Western Blot. Also, there was very little background. Overall, very happy with this product.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HGSOC Commercial Cell Line