EPCAM/CD326 Polyklonaler Antikörper

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• KD/KO Validated

EPCAM/CD326 Polyklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-21050

Synonyme

EPCAM, EGP, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1

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  IF Staining of MCF-7 using CL488-21050

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 EPCAM/CD326 antibody (CL488-21050) at dilution of 1:200.

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  FC experiment of MCF-7 using CL488-21050

  1x10^6 MCF-7 cells were surface stained with 0.8 ug CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclonal antibody (CL488-21050) (red), or 0.8 ug Isotype Control (blue). Cells were not fixed.

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  FC experiment of A431 using CL488-21050

  1x10^6 A431 cells were surface stained with 0.4 ug CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclonal antibody (CL488-21050) (red), or 0.4 ug Control Antibody (blue). Cells were not fixed.

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  FC experiment of HT-29 using CL488-21050

  1x10^6 HT-29 cells were surface stained with 0.8 ug CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclonal antibody (CL488-21050) (red), or 0.8 ug Control Antibody (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen, A431-Zellen, HT-29-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-21050 bindet in IF/ICC, FC EPCAM/CD326 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EPCAM/CD326 fusion protein Ag15393
• Vollständiger Name: epithelial cell adhesion molecule
• Berechnetes Molekulargewicht: 314 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC014785
• Gene symbol: EPCAM
• Gene ID (NCBI): 4072
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)