EPHA1-specific Monoklonaler Antikörper

EPHA1-specific Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1B7C3

Kat-Nr. : 60155-2-Ig

Synonyme

EPH, EPH receptor A1, EPHA1, Ephrin type A receptor 1, EPHT, EPHT1

---

• 

  WB analysis of HeLa using 60155-2-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 60155-2-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60155-2-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 60155-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60155-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60155-2-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60155-2-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60155-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60155-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

60155-2-Ig bindet in WB, IHC, ELISA EPHA1-specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: EPH receptor A1
• Berechnetes Molekulargewicht: 108 kDa
• Beobachtetes Molekulargewicht: 108 kDa
• GenBank-Zugangsnummer: BC130291
• Gene symbol: EPHA1
• Gene ID (NCBI): 2041
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPHA1, also named as EPH, EPHT and EPHT1, belongs to ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction.

Publikationen

Species	Application	Title
human	WB	Metab Brain Dis; Sequencing and Bioinformatics analysis of lncRNA/circRNA-miRNA-mRNA in Glioblastoma multiforme; Authors - Renjie Wang