EPS15R Polyklonaler Antikörper

EPS15R Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 21243-1-AP

Synonyme

EPS15L1, Eps15-related protein, Eps15 related protein, Epidermal growth factor receptor substrate 15-like 1

---

• 

  WB analysis using 21243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21243-1-AP (EPS15R antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21243-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21243-1-AP (EPS15R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 21243-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21243-1-AP (EPS15R antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 21243-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21243-1-AP (EPS15R antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of BxPC-3 using 21243-1-AP

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using 21243-1-AP (EPS15R antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HEK-293-Zellen, MCF-7-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

21243-1-AP bindet in WB, IHC, IF/ICC, ELISA EPS15R und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EPS15R fusion protein Ag15735
• Vollständiger Name: epidermal growth factor receptor pathway substrate 15-like 1
• Berechnetes Molekulargewicht: 864 aa, 94 kDa
• Beobachtetes Molekulargewicht: 94 kDa
• GenBank-Zugangsnummer: BC142716
• Gene symbol: EPS15R
• Gene ID (NCBI): 58513
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPS15R is a protein that belongs to the Eps15 family of proteins, which are known for their roles in intracellular trafficking, particularly in the regulation of endocytosis and vesicle formation. EPS15R, like other members of the Eps15 family, functions as a scaffolding adaptor protein and is involved in both secretion and endocytosis. It has been shown to bind to AP-1 and AP-2 complexes, inositol lipids, and several other proteins that are crucial for the regulation of intracellular trafficking. EPS15R is also implicated in the regulation of membrane morphology, which is essential for intracellular vesicle formation and trafficking . It has been detected in the nucleus of mammalian cells, and its activation through growth factor receptors induces tyrosine phosphorylation and mono-ubiquitination, although the specific roles of these post-translational modifications are not yet fully understood

Publikationen

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
pig	WB	Emerg Microbes Infect; CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis; Authors - Xin Chen