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HER2/ErbB2 Polyklonaler Antikörper

HER2/ErbB2 Polyklonal Antikörper für WB, IHC, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(81)

Kat-Nr. : 18299-1-AP

Synonyme

ERBB2, CD340, HER 2, HER 2/neu, HER2



Geprüfte Anwendungen

Erfolgreiche Detektion in WB4T1-Zellen, HeLa-Zellen, MCF-7-Zellen, SGC-7901-Zellen
Erfolgreiche IPSGC-7901-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, human ovary cancer tissue
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-Fromouse breast cancer

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:12000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:300-1:1200
Immunfluoreszenz (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

18299-1-AP bindet in WB, IHC, IF-Fro, IP, ELISA HER2/ErbB2 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Berechnetes Molekulargewicht 138 kDa
Beobachtetes Molekulargewicht 185 kDa
GenBank-ZugangsnummerBC167147
Gene symbol HER2/ErbB2
Gene ID (NCBI) 2064
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas. This antibody raised against a synthesized peptide corresponding to 1237-1255aa of human HER2 recognizes the 185-kDa full-length glycosylated form and other lower molecular-mass forms of HER2, including the truncated form.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for HER2/ErbB2 antibody 18299-1-APProtokoll herunterladen
IHC protocol for HER2/ErbB2 antibody 18299-1-APProtokoll herunterladenl
IF protocol for HER2/ErbB2 antibody 18299-1-APProtokoll herunterladen
IP protocol for HER2/ErbB2 antibody 18299-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Signal Transduct Target Ther

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

Authors - Chuan Yang
humanWB

Cell Metab

Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity

Authors - Zilong Zhang
humanIHC

Cell Res

Protein C receptor is a therapeutic stem cell target in a distinct group of breast cancers.

Authors - Daisong Wang
mouseWB

Small

Efficient Delivery of GSDMD-N mRNA by Engineered Extracellular Vesicles Induces Pyroptosis for Enhanced Immunotherapy

Authors - Yuqi Xing
humanIHC

Nat Commun

Targeting 17q23 amplicon to overcome the resistance to anti-HER2 therapy in HER2+ breast cancer.

Authors - Yunhua Liu
human

J Nanobiotechnology

Accurate HER2 determination in breast cancer: a prominent COF-immobilized enzyme-enhanced electrochemical aptasensor employing 4-acetamidophenol as an efficient mediator

Authors - Yue Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Vanina (Verified Customer) (07-28-2021)

MDA-MB-453 cell line was transfected with different concentrations of HER2/Erbb2 siRNA for 48h. Western blot using HER2/Erbb2 rabbit Proteintech antibody (18299-1-AP) at 1:2,000 dillution confirms the HER2/Erbb2 knockdown.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2,000
  • Cell Tissue Type: MDA-MB-453 breast cancer cell line
HER2/ErbB2 Antibody Western Blot validation (1:2,000 dilution) in MDA-MB-453 breast cancer cell line (Cat no:18299-1-AP)