ERBB2IP Monoklonaler Antikörper

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• KD/KO Validated

ERBB2IP Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A8D4

Kat-Nr. : 68159-1-Ig

Synonyme

Densin 180 like protein, erbb2 interacting protein, ERBB2IP, ERBIN, KIAA1225, LAP2, Protein LAP2

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Galerie der Validierungsdaten

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  WB analysis using 68159-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68159-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 68159-1-Ig

  WB result of ERBB2IP antibody (68159-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERBB2IP transfected HeLa cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, A549-Zellen, HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, LNCaP-Zellen, MCF-7-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68159-1-Ig bindet in WB, ELISA ERBB2IP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ERBB2IP fusion protein Ag18514
• Vollständiger Name: erbb2 interacting protein
• Berechnetes Molekulargewicht: 1412 aa, 158 kDa
• Beobachtetes Molekulargewicht: 150-180 kDa
• GenBank-Zugangsnummer: BC115012
• Gene symbol: ERBB2IP
• Gene ID (NCBI): 55914
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERBB2IP is a cytoplasmic protein that contains leucine-rich repeats (LRR) and PSD95/Dlg1/zo-1 (PDZ) domain (thus named a LAP protein). ERBB2IP interacts, via its single PDZ domain, specifically with ErbB2, but not with ErbB3, ErbB4, or EGFR. It is colocalized with ErbB2 at the basolateral membranes of epithelial cells. In addition, ERBB2IP also interacts with PSD‐95, Lano and with three p120‐related catenins, eBPAG1 and the β4 subunit of the α6β4 integrin, two major components of hemidesmosomes. The molecular weight of ERBB2IP was predicted 150~180 kDa by Western Blot.(PMID: 25288731,PMID: 24252174,PMID: 19289088)