ERBB4 Polyklonaler Antikörper

ERBB4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 19943-1-AP

Synonyme

4ICD, E4ICD, ERBB4, HER4, p180erbB4, s80HER4

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 19943-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19943-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19943-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19943-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 19943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 19943-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse lung using 19943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19943-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse heart using 19943-1-AP

  Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (green) and ErbB4 (red, Cat. No 19943-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 488 secondary antibody. ErbB4 is a key member of ErbB signaling and involved in development and was visualized with an Alexa Fluor 555 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Mauslungengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

19943-1-AP bindet in WB, IHC, IF, ELISA ERBB4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
• Berechnetes Molekulargewicht: 147 kDa
• Beobachtetes Molekulargewicht: 180 kDa, 147 kDa
• GenBank-Zugangsnummer: NM_001042599
• Gene symbol: ERBB4
• Gene ID (NCBI): 2066
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERBB4, also named p180erbB4 and HER4, belongs to the protein kinase superfamily, Tyr protein kinase family, and EGF receptor subfamily. ERBB4 specifically binds and it is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin, and NTAK. Interaction with these factors induces cell differentiation. ERBB4 is not activated by EGF, TGF-A, and amphiregulin. ERBB4 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody recognizes the C-term of ERBB4. Alternatively spliced variants that encode different protein isoforms with a calculated molecular weight of 144-147 kDa have been described. The apparent molecular weight of full-length ERBB4 is 150-200 kDa due to glycosylation and phosphorylation. Full-length ERBB4 can be cleaved into an 80-kDa ERBB4 intracellular fragment and a 120-kDa extracellular fragment (PMID: 11343971; 16251361).

Publikationen

Species	Application	Title
mouse	IF	Adv Sci (Weinh); Neuregulin4 Acts on Hypothalamic ErBb4 to Excite Oxytocin Neurons and Preserve Metabolic Homeostasis; Authors - Yi Zhang
human	IHC	Respir Res; Identification of targetable kinases in idiopathic pulmonary fibrosis.; Authors - Hisao Higo
human	WB	Front Oncol; DNAJC12 as a Mediator Between ESR1 and ERBB4 in Breast Carcinoma Cells.; Authors - Mianjie Lin
pig	WB	J Neuroinflammation; EGFR transactivation contributes to neuroinflammation in Streptococcus suis meningitis.; Authors - Xiao-Pei Yang
human	WB,IF	Cell Mol Neurobiol; Identifying Genes that Affect Differentiation of Human Neural Stem Cells and Myelination of Mature Oligodendrocytes; Authors - Dou Ye
mouse	WB	DNA Cell Biol; AK003290 Protects Myocardial Cells Against Apoptosis and Promotes Cardiac Function Recovery Via miR-539-3p/ErbB4 Axis in Ischemic-Reperfusion Injury.; Authors - Yong Tang

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