ERCC2 Polyklonaler Antikörper

ERCC2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 10818-1-AP

Synonyme

BTF2 p80, COFS2, CXPD, EM9, ERCC2, TFIIH 80 kDa subunit, TFIIH p80, TTD, XPD, XPDC

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10818-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10818-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 10818-1-AP

  IP Result of anti-ERCC2 (IP:10818-1-AP, 5ug; Detection:10818-1-AP 1:500) with K-562 cells lysate 8000ug.

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  IHC staining of human lymphoma using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human lymphoma using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 5 publications below

Produktinformation

10818-1-AP bindet in WB, IP, IHC, ELISA ERCC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERCC2 fusion protein Ag1038
• Vollständiger Name: excision repair cross-complementing rodent repair deficiency, complementation group 2
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC008346
• Gene symbol: ERCC2
• Gene ID (NCBI): 2068
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Excision-repair complementing defective in Chinese hamster 2(ERCC2), belongs to the nucleotide excision repair pathway, is a component of the TFIIH transcription complex and required for the association of the CDK-activating kinase(CAK) subcomplex with TFIIH. Since TFIIH is involved in both DNA repair and cell cycle progression, ERCC2 is implicated to play an integral role in the nucleotide excision repair pathway. Also it involves in the regulation of vitamin-D receptor activity. As a part of the mitotic spindle-associated MMXD complex, ERCC2 has a role in chromosome segregation.

Publikationen

Species	Application	Title
human	IHC	Am J Cancer Res; Predictive value of ERCC1, ERCC2, ERCC4, and glutathione S-Transferase Pi expression for the efficacy and safety of FOLFIRINOX in patients with unresectable pancreatic cancer.; Authors - Shun Tezuka
human	IHC	Methods; Synchronous detection of miRNAs, their targets and downstream proteins in transferred FFPE sections: applications in clinical and basic research.; Authors - Zhao Jin-yao JY
human	IHC	Cancer Sci; Very low prevalence of XPD K751Q polymorphism and its association with XPD expression and outcomes of FOLFOX-4 treatment in Asian patients with colorectal carcinoma.; Authors - Lai Jiun-I JI
human	WB	Oncotarget; PMID: 27449101; Authors - Henan Zhao
human	IHC	Biomarkers; Aberrant methylation of nucleotide excision repair genes is associated with chronic arsenic poisoning; Authors - Aihua Zhang
human	IHC	J Transl Med; Proteomic analysis predicts anti-angiogenic resistance in recurred glioblastoma; Authors - Hanwool Jeon

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zizhao (Verified Customer) (08-15-2018)

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1: 1000
• Cell Tissue Type: human keratinocytes