ERCC6/CSB Polyklonaler Antikörper

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• KD/KO Validated

ERCC6/CSB Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 24291-1-AP

Synonyme

ERCC6, ARMD5, ATP dependent helicase ERCC6, Chimeric CSB-PGBD3 protein, Chimeric ERCC6-PGBD3 protein

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  WB analysis using 24291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24291-1-AP (ERCC6/CSB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24291-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24291-1-AP (ERCC6/CSB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24291-1-AP

  IP result of anti-ERCC6/CSB (IP:24291-1-AP, 4ug; Detection:24291-1-AP 1:300) with HeLa cells lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HeLa-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

24291-1-AP bindet in WB, IF, IP, ELISA ERCC6/CSB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERCC6/CSB fusion protein Ag18009
• Vollständiger Name: excision repair cross-complementing rodent repair deficiency, complementation group 6
• Berechnetes Molekulargewicht: 1493 aa, 168 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC127104
• Gene symbol: ERCC6/CSB
• Gene ID (NCBI): 2074
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; A Gene Expression Signature to Predict Nucleotide Excision Repair Defects and Novel Therapeutic Approaches.; Authors - Rongbin Wei
mouse	WB,IF	Front Mol Biosci; Blockage of ERCC6 Alleviates Spinal Cord Injury Through Weakening Apoptosis, Inflammation, Senescence, and Oxidative Stress.; Authors - Peng Zou ; KD Validated
human	WB	Life Sci Alliance; Elevated glucose increases genomic instability by inhibiting nucleotide excision repair.; Authors - Alexandra K Ciminera
human	WB,IP	Nucleic Acids Res; Conserved intronic secondary structures with concealed branch sites regulate alternative splicing of poison exons; Authors - Hao Li