ERI1 Polyklonaler Antikörper

ERI1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14592-1-AP

Synonyme

3 5 exonuclease ERI1, 3 5 exoribonuclease 1, 3EXO, 3'HEXO, Eri 1 homolog, ERI1, exoribonuclease 1, HEXO, Histone mRNA 3 exonuclease 1, Protein 3hExo, THEX1

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 14592-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14592-1-AP (ERI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 14592-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14592-1-AP (ERI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14592-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14592-1-AP (ERI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14592-1-AP (ERI1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 14592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14592-1-AP (ERI1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14592-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14592-1-AP (ERI1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14592-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14592-1-AP (ERI1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 14592-1-AP

  Immunofluorescent analysis of HepG2 cells using 14592-1-AP (ERI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HepG2-Zellen, HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14592-1-AP bindet in WB, IHC, IF,ELISA ERI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERI1 fusion protein Ag6125
• Vollständiger Name: exoribonuclease 1
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC035279
• Gene symbol: ERI1
• Gene ID (NCBI): 90459
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERI1, also named as HEXO, 3'HEXO and THEX1, contains one exonuclease domain and one SAP domain. The SAP domain is necessary for binding to the stem-loop structure of histone mRNAs and to form the ternary complex with SLBP, but not for 3'-end histone mRNA exonuclease activity. It is a RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. It is able to bind other mRNAs. ERI1 is required for 5.8S rRNA 3'-end processing. It also binds to 5.8s ribosomal RNA. ERI1 binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.