ERK1/2 Monoklonaler Antikörper

ERK1/2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(51)

CloneNo.

4G8A11

Kat-Nr. : 66192-1-Ig

Synonyme

ERK, ERK1, ERK2, 4G8A11, EC:2.7.11.24

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  WB analysis using 66192-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66192-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66192-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66192-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 66192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human cervical cancer using 66192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human ovary tumor using 66192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human ovary tumor using 66192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IF Staining of HeLa using 66192-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ERK1/2 antibody (66192-1-Ig, Clone: 4G8A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:2000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 48 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66192-1-Ig bindet in WB, IHC, IF/ICC, CoIP, ELISA ERK1/2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ERK1/2 fusion protein Ag20350
• Vollständiger Name: mitogen-activated protein kinase 3
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 38-44 kDa
• GenBank-Zugangsnummer: BC013992
• Gene symbol: ERK1
• Gene ID (NCBI): 5595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Publikationen

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Ccl2-Induced Regulatory T Cells Balance Inflammation Through Macrophage Polarization During Liver Reconstitution; Authors - Rui Wang
mouse	CoIP	PLoS Biol; mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.; Authors - Zi-Yang Zhang
human	WB	Oncogene; ALK fusion promotes metabolic reprogramming of cancer cells by transcriptionally upregulating PFKFB3; Authors - Mengnan Hu
human	WB	Stem Cells; miR-223 regulates adipogenic and osteogenic differentiation of mesenchymal stem cells through a C/EBPs/miR-223/FGFR2 regulatory feedback loop.; Authors - Xiaohui Guan
human,mouse	WB	Front Immunol; Enhancing pancreatic cancer treatment: the role of H101 oncolytic virus in irreversible electroporation; Authors - Pu Xi
mouse	WB	Diabetes; Pygo2 Regulates Adiposity and Glucose Homeostasis via β-Catenin-Axin2-GSK3β Signaling Pathway.; Authors - Yuan-Yuan Xie