ERK1/2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ERK1/2 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

231

Rezensionen (1)

Kat-Nr. : 11257-1-AP

Synonyme

ERK, ERK 1, ERK 1/2, ERK1, ERK1/2, ERK2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

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Galerie der Validierungsdaten

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  WB analysis using 11257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1/2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11257-1-AP

  WB result of ERK1/2 antibody (11257-1-AP, 1:1000) with HeLa and HEK293 cells.

• 

  WB analysis of HeLa using 11257-1-AP

  WB result of ERK1/2 antibody (11257-1-AP, 1:2000) with si-Control and si-ERK1/2 transfected HeLa cells.

• 

  IHC staining of human colon cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A375 using 11257-1-AP

  Immunofluorescent analysis of A375 cells using 11257-1-AP (ERK1/2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 11257-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11257-1-AP (ERK1/2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11257-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ERK1/2 (11257-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Mauslungengewebe, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A375-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 227 publications below
• IHC: See 4 publications below
• IF: See 8 publications below

Produktinformation

11257-1-AP bindet in WB, IHC, IF, FC, ELISA ERK1/2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERK1/2 fusion protein Ag1759
• Vollständiger Name: mitogen-activated protein kinase 3
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 38-44 kDa
• GenBank-Zugangsnummer: BC013992
• Gene symbol: MAPK3
• Gene ID (NCBI): 5595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Publikationen

Species	Application	Title
	WB	Cancer Res; Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells; Authors - Li Xia
mouse	WB	ACS Appl Mater Interfaces; Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels.; Authors - Chaoqun Li
mouse	WB	J Nanobiotechnology; Peptide-anchored neutrophil membrane-coated biomimetic nanodrug for targeted treatment of rheumatoid arthritis; Authors - Ni Yang
human	WB	Br J Pharmacol; Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A; Authors - Lu Zhang
human	WB	Pharmacol Res; Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex; Authors - Li Gu
	WB	Cell Rep; Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation; Authors - Zehe Ge

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

XB (Verified Customer) (10-29-2020)

A reliable ab to detect total ERK1/2 by Western blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain

ERK1/2 Antibody Western Blot validation (1:3000 dilution) in mouse brain (Cat no:11257-1-AP)