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ERK1/2 Polyklonaler Antikörper
ERK1/2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (6)
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 11257-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | PC-12-Zellen, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Mauslungengewebe, NIH/3T3-Zellen | 
| Erfolgreiche IP | HEK-293-Zellen | 
| Erfolgreiche Detektion in IHC | humanes Kolonkarzinomgewebe,  humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF/ICC | HEK-293-Zellen | 
| Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunhistochemie (IHC) | IHC : 1:100-1:400 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 | 
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 481 publications below | 
| IHC | See 11 publications below | 
| IF | See 15 publications below | 
| CoIP | See 2 publications below | 
Produktinformation
11257-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA ERK1/2 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte | 
| In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Hund, Maus, Ratte, Rind, Zebrafisch, Ziege | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | ERK1/2 fusion protein Ag1759 | 
| Vollständiger Name | mitogen-activated protein kinase 3 | 
| Berechnetes Molekulargewicht | 43 kDa | 
| Beobachtetes Molekulargewicht | 38-44 kDa | 
| GenBank-Zugangsnummer | BC013992 | 
| Gene symbol | ERK1 | 
| Gene ID (NCBI) | 5595 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for ERK1/2 antibody 11257-1-AP | Protokoll herunterladen | 
| IHC protocol for ERK1/2 antibody 11257-1-AP | Protokoll herunterladenl | 
| IF protocol for ERK1/2 antibody 11257-1-AP | Protokoll herunterladen | 
| IP protocol for ERK1/2 antibody 11257-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Nat Commun Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer | ||
| Biomaterials Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure | ||
| Small Biomimetic Nanoplatform Integrating CeO₂ Nanozymes and Anti-Inflammatory Peptides for Osteoarthritis Therapy | ||
| Redox Biol Twin defect-rich Pt ultrathin nanowire nanozymes alleviate inflammatory skin diseases by scavenging reactive oxygen species | ||
| Cancer Res Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells | ||
| EMBO Mol Med Luteolin detoxifies DEHP and prevents liver injury by degrading Uroc1 protein in mice | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Charlotte (Verified Customer) (08-15-2024) | The company suggests us this ERK ab as the other one we bought from them was not working. I overlaid ERK with pERK and it matches nicely. Satisfied of this antibody. Done in milk. 
  | 
| FH XB (Verified Customer) (10-29-2020) | A reliable ab to detect total ERK1/2 by Western blotting. 
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