ERLIN1 Polyklonaler Antikörper

ERLIN1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17311-1-AP

Synonyme

C10orf69, ER lipid raft associated 1, Erlin 1, ERLIN1, KE04, KEO4, Protein KE04, SPFH1

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Galerie der Validierungsdaten

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  WB analysis using 17311-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 17311-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human ovary cancer using 17311-1-AP

  human ovary cancer were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 17311-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17311-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17311-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 17311-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 17311-1-AP

  IP Result of anti-ERLIN1 (IP:17311-1-AP, 3ug; Detection:17311-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse liver using 17311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17311-1-AP (ERLIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 17311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17311-1-AP (ERLIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17311-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17311-1-AP (ERLIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17311-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17311-1-AP (ERLIN1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 17311-1-AP

  Immunofluorescent analysis of HeLa cells using 17311-1-AP (ERLIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, COLO 320-Zellen, humane Ovarialkarzinomzellen, humanes Hirngewebe, humanes Nierengewebe, Mauslebergewebe, Ratten-Pankreasgewebe, U-251-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

17311-1-AP bindet in WB, IP, IHC, IF, ELISA ERLIN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERLIN1 fusion protein Ag11279
• Vollständiger Name: ER lipid raft associated 1
• Berechnetes Molekulargewicht: 346 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC031791
• Gene symbol: ERLIN1
• Gene ID (NCBI): 10613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Cell; BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.; Authors - Fabien Llambi
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex; Authors - Michael L van de Weijer
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.; Authors - Michael L van de Weijer
human	WB	Elife; RNF41 regulates the damage recognition receptor Clec9A and antigen cross-presentation in mouse dendritic cells.; Authors - Kirsteen M Tullett
human	WB	PLoS Genet; Disruption of the ERLIN-TM6SF2-APOB complex destabilizes APOB and contributes to non-alcoholic fatty liver disease.; Authors - Bo-Tao Li