ERO1LB Polyklonaler Antikörper

ERO1LB Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18312-1-AP

Synonyme

ERO1 L beta, ERO1 like beta (S. cerevisiae), ERO1 like protein beta, ERO1B, ERO1LB, Oxidoreductin 1 L beta

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Galerie der Validierungsdaten

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  WB analysis of human brain using 18312-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 18312-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18312-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 18312-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18312-1-AP (ERO1LB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 18312-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18312-1-AP (ERO1LB antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18312-1-AP bindet in WB, IHC,ELISA ERO1LB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ERO1-like beta (S. cerevisiae)
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC032823
• Gene symbol: ERO1LB
• Gene ID (NCBI): 56605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERO1LB is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. It is required for the folding of cell, thereby being a source of oxidative stress. This antibody has no cross reaction to ERO1L.