ESRRG Polyklonaler Antikörper

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• KD/KO Validated

ESRRG Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 14017-1-AP

Synonyme

Estrogen-related receptor gamma, Estrogen receptor-related protein 3, ERRG2, ERR3, ERR gamma-2

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  WB analysis of human spleen using 14017-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 14017-1-AP (ESRRG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 14017-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 14017-1-AP (ESRRG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14017-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14017-1-AP (ESRRG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14017-1-AP (ESRRG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 14017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14017-1-AP (ESRRG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14017-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14017-1-AP (ESRRG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 14017-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 14017-1-AP (ESRRG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 14017-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 14017-1-AP (ESRRG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Milzgewebe, humanes Nierengewebe
• Erfolgreiche Detektion in IHC: Ratten-Magengewebe, Maushirngewebe, Mausherzgewebe, Rattenhirngewebe, Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14017-1-AP bindet in WB, IHC, IF, ELISA ESRRG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ESRRG fusion protein Ag5127
• Vollständiger Name: estrogen-related receptor gamma
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC064700
• Gene symbol: ESRRG
• Gene ID (NCBI): 2104
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Estrogen-related receptor gamma (ERR-gamma), also known as NR3B3 (nuclear receptor subfamily 3, group B, member 3), is a member of nuclear hormone receptor family of steroid hormone receptors. As an orphan nuclear receptor, it binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements. It behaves as a constitutive activator of transcription. 4-hydroxytamoxifen and diethylstilbestrol may act as inverse agonists and deactivate ESRRG. It also seems to be the target of bisphenol A. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human ESRRG.

Publikationen

Species	Application	Title
human	IHC,IF	Acta Neuropathol Commun; Nucleolar stress in C9orf72 and sporadic ALS spinal motor neurons precedes TDP-43 mislocalization.; Authors - Olubankole Aladesuyi Arogundade
human	WB	J Transl Med; ESRRG-PKM2 axis reprograms metabolism to suppress esophageal squamous carcinoma progression and enhance anti-PD-1 therapy efficacy; Authors - Tianxiao Wang
rat	WB	J Ethnopharmacol; Atractylodes macrocephala Rhizoma alleviates blood hyperviscosity induced by high-fat, high-sugar, and high-salt diet via inhibition of gut-liver inflammatory activation and fibrinogen synthesis; Authors - Qiwei Lin
human	WB	Mol Cancer Res; Integrative Epigenetic and Gene Expression Analysis of Renal Tumor Progression to Metastasis.; Authors - Hye-Young Nam
mouse	WB	Int J Biochem Cell Biol; Estrogen-related receptor (ERR) γ protects against puromycin aminonucleoside-induced podocyte apoptosis by targeting PI3K/Akt signaling.; Authors - Wei Gong ; KD Validated
mouse	WB	Front Pharmacol; Tanshinone IIA delays liver aging by modulating oxidative stress; Authors - Qi Liu