ETFB Polyklonaler Antikörper

ETFB Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 17925-1-AP

Synonyme

Beta-ETF, FP585, MADD

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  WB analysis of mouse skeletal muscle using 17925-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17925-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17925-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17925-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17925-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 17925-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17925-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17925-1-AP

  IP result of anti-ETFB (IP:17925-1-AP, 3ug; Detection:17925-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human stomach cancer using 17925-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17925-1-AP (ETFB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17925-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETFB antibody (17925-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, humanes Herzgewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 2 publications below

Produktinformation

17925-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ETFB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ETFB fusion protein Ag12344
• Vollständiger Name: electron-transfer-flavoprotein, beta polypeptide
• Berechnetes Molekulargewicht: 255 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC093961
• Gene symbol: ETFB
• Gene ID (NCBI): 2109
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kDa alpha subunits (ETFA)and 28 kDa beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer.It serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.ETFB has 2 isoforms produced by alternative splicing. Defects in ETFB are the cause of glutaric aciduria type 2B (GA2B).

Publikationen

Species	Application	Title
human	IF	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Mol Cell; PrimPol, an archaic primase/polymerase operating in human cells.; Authors - Sara García-Gómez
human	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
human	WB	Mol Cell Biol; A mutation in the FAD-dependent oxidoreductase FOXRED1 results in cell-type specific assembly defects in oxidative phosphorylation complexes I and II.; Authors - Olga Zurita Rendón
human	WB	J Biol Chem; Human METTL20 Methylates Lysine Residues Adjacent to the Recognition Loop of the Electron Transfer Flavoprotein in Mitochondria.; Authors - Virginie F Rhein
human	WB	J Cell Sci; Mitochondrial maintenance under oxidative stress depends on mitochondrial but not nuclear α isoform of OGG1.; Authors - Debora Lia