ETS1 Monoklonaler Antikörper

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• KD/KO Validated

ETS1 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

4F2D4

Kat-Nr. : 66598-1-Ig

Synonyme

ETS 1, ETS1, EWSR2, p54, Protein C ets 1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 66598-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66598-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 66598-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66598-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 66598-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETS1 antibody (66598-1-Ig, Clone: 4F2D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Daudi-Zellen, HSC-T6-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:5000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

66598-1-Ig bindet in WB, IHC, IF, ELISA ETS1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ETS1 fusion protein Ag2127
• Vollständiger Name: v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
• Berechnetes Molekulargewicht: 441 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC017314
• Gene symbol: ETS1
• Gene ID (NCBI): 2113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo.

Publikationen

Species	Application	Title
human	WB,IHC	Mol Med; ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy.; Authors - Lihong Lu ; KD Validated
human	WB	Blood Adv; ETS1 is a novel transcriptional regulator of adult T-cell leukemia/lymphoma of North American descent; Authors - Rebecca A Luchtel