ETS1 Monoklonaler Antikörper

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• KD/KO Validated

ETS1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

4F2D4

Kat-Nr. : 66598-1-Ig

Synonyme

Protein C-ets-1, EWSR2, ETS 1, 4F2D4

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  WB analysis of HepG2 using 66598-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66598-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 66598-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66598-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat spleen using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 66598-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETS1 antibody (66598-1-Ig, Clone: 4F2D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Daudi-Zellen, HSC-T6-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Rattenmilzgewebe, Mauslungengewebe, Maus-Dünndarmgewebe, Rattenlungengewebe, Ratten-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:5000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

66598-1-Ig bindet in WB, IHC, IF/ICC, ChIP, ELISA ETS1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ETS1 fusion protein Ag2127
• Vollständiger Name: v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
• Berechnetes Molekulargewicht: 441 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC017314
• Gene symbol: ETS1
• Gene ID (NCBI): 2113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; PLA2G7 promotes immune evasion of bladder cancer through the JAK-STAT-PDL1 axis; Authors - Ding Peng
human	WB,IHC	Mol Med; ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy.; Authors - Lihong Lu ; KD Validated
human	WB	Blood Adv; ETS1 is a novel transcriptional regulator of adult T-cell leukemia/lymphoma of North American descent; Authors - Rebecca A Luchtel
human	WB	Nat Microbiol; FEAR antiviral response pathway is independent of interferons and countered by poxvirus proteins; Authors - Emily A Rex
human	WB,IHC,ChIP	Int J Biol Sci; ETS1-mediated Regulation of SOAT1 Enhances the Malignant Phenotype of Oral Squamous Cell Carcinoma and Induces Tumor-associated Macrophages M2-like Polarization; Authors - Yueying Liu
human,mouse	WB	Elife; Neurons enhance blood-brain barrier function via upregulating claudin-5 and VE-cadherin expression due to glial cell line-derived neurotrophic factor secretion; Authors - Lu Yang ; KD Validated