ETV5 Polyklonaler Antikörper

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• KD/KO Validated

ETV5 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Kat-Nr. : 13011-1-AP

Synonyme

ERM, Ets related protein ERM, ETS translocation variant 5, ets variant 5, ETV5

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Galerie der Validierungsdaten

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  WB analysis using 13011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13011-1-AP (ETV5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13011-1-AP (ETV5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13011-1-AP (ETV5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13011-1-AP (ETV5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 13011-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 13011-1-AP (ETV5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, PC-3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 9 publications below
• CoIP: See 1 publications below
• ChIP: See 4 publications below

Produktinformation

13011-1-AP bindet in WB, IHC, IF, CoIP, ChIP, ELISA ETV5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ETV5 fusion protein Ag3708
• Vollständiger Name: ets variant 5
• Berechnetes Molekulargewicht: 510 aa, 58 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC007333
• Gene symbol: ETV5
• Gene ID (NCBI): 2119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney

Publikationen

Species	Application	Title
human	WB	Cell; Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription.; Authors - Jaehong Suh
human	ChIP	Nat Genet; The dynamic, combinatorial cis-regulatory lexicon of epidermal differentiation.; Authors - Daniel S Kim
mouse	WB	Nat Commun; A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility; Authors - Daiji Kiyozumi
mouse	IF	Nat Commun; A critical role for NF2 and the Hippo pathway in branching morphogenesis.; Authors - Antoine Reginensi
mouse	IF, ChIP	Proc Natl Acad Sci U S A; Transcription factor Etv5 is essential for the maintenance of alveolar type II cells.; Authors - Zhen Zhang
mouse	IF	PLoS Biol; Ret and Etv4 Promote Directed Movements of Progenitor Cells during Renal Branching Morphogenesis.; Authors - Paul Riccio