ETV6 Monoklonaler Antikörper

ETV6 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A5A2

Kat-Nr. : 67579-1-Ig

Synonyme

ETS related protein Tel1, ETS translocation variant 6, ets variant 6, ETV6, TEL, TEL/ABL, TEL1, Transcription factor ETV6

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 67579-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67579-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 67579-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 67579-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 67579-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67579-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67579-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67579-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67579-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A431-Zellen, K-562-Zellen, THP-1-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67579-1-Ig bindet in WB, IHC, ELISA ETV6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ETV6 fusion protein Ag30054
• Vollständiger Name: ets variant 6
• Berechnetes Molekulargewicht: 452 aa, 53 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC043399
• Gene symbol: ETV6
• Gene ID (NCBI): 2120
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ETV6(ETS Variant Transcription Factor 6), also known as TEL, is a Protein Coding gene. ETV6 is a member of the ETS family of transcription factors encoded by the ETS variant 6 gene (ETV6), located on chromosome 12p13.2. ETV6 protein contains 3 domains: a) the pointed N-terminal (i.e. PNT) domain which forms oligomer partners with itself as well as other transcription factors (e.g. FLI1) and is required for ETV6's transcriptional repressing activity; b) the central regulatory domain; and c) the C-terminal DNA-binding domain(PMID: 22578774). ETV6 is highly expressed in early hematopoietic progenitor cells(HPCs) and is essential for hematopoiesis in the bone marrow(PMID: 31248877).