EXOSC10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EXOSC10 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 16731-1-AP

Synonyme

Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p

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  WB analysis using 16731-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16731-1-AP (EXOSC10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16731-1-AP

  WB result of EXOSC10 antibody (16731-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC10 transfected HeLa cells.

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  WB analysis of mouse spleen using 16731-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16731-1-AP (EXOSC10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16731-1-AP

  IP result of anti-EXOSC10 (IP:16731-1-AP, 4ug; Detection:16731-1-AP 1:700) with HeLa cells lysate 1200ug.

• 

  IHC staining of human stomach cancer using 16731-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 16731-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 16731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 16731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16731-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC10 antibody (16731-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, Mausmilzgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

16731-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA EXOSC10 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXOSC10 fusion protein Ag10189
• Vollständiger Name: exosome component 10
• Berechnetes Molekulargewicht: 885 aa, 101 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC073788
• Gene symbol: EXOSC10
• Gene ID (NCBI): 5394
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

Publikationen

Species	Application	Title
human	WB,IHC	PeerJ; EXOSC10 is a novel hepatocellular carcinoma prognostic biomarker: a comprehensive bioinformatics analysis and experiment verification; Authors - Zhi-Yong Meng ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (12-17-2023)

Antibody recognise Exosc10 band and some other non-specific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

EXOSC10 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:16731-1-AP)