EXOSC6 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

EXOSC6 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241535C3

Kat-Nr. : 84346-4-RR

Synonyme

241535C3, EAP4, exosome component 6, hMtr3, hMtr3p

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  WB analysis using 84346-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84346-4-RR (EXOSC6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 84346-4-RR

  WB result of EXOSC6 antibody (84346-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC6 transfected HEK-293 cells.

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  IF Staining of HeLa using 84346-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC6 antibody (84346-4-RR, Clone: 241535C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 84346-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug EXOSC6 Recombinant antibody (84346-4-RR, Clone:241535C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84346-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84346-4-RR against Human EXOSC6 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HaCaT-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84346-4-RR bindet in WB, IF/ICC, FC (Intra), ELISA EXOSC6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EXOSC6 fusion protein Ag33513
• Vollständiger Name: exosome component 6
• Berechnetes Molekulargewicht: 28kd
• Beobachtetes Molekulargewicht: 28-32 kDa
• GenBank-Zugangsnummer: NM_058219
• Gene symbol: EXOSC6
• Gene ID (NCBI): 118460
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXOSC6 (exosome component 6), also known as p11 or MTR3. The calculated molecular weight of EXOSC6 is 28 kDa. And it has low tissue specificity. The gene product constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. It is also a component of the RNA exosome complex (PMID: 29906447). The composition of human exosome is similar to its yeast counterpart. EXOSC6 is homologous to the yeast Mtr3 protein. Its exact function is not known, however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation.