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Endoglin/CD105 Monoklonaler Antikörper

Endoglin/CD105 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D5E8

Kat-Nr. : CL488-67075

Synonyme

CD105, END, endoglin, Endoglin/CD105, ENG, FLJ41744, HHT1, ORW, ORW1

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of Human liver cancer tissue using CoraLite® Plus 488-conjugated Endoglin/CD105 Monoc (CL488-67075)

    IF Staining of Human liver cancer using CL488-67075

    Immunofluorescent analysis of (4% PFA) fixed Human liver cancer tissue using CoraLite® Plus 488 Endoglin/CD105 antibody (CL488-67075, Clone: 2D5E8 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of Human liver cancer tissue using CoraLite® Plus 488-conjugated Endoglin/CD105 Monoc (CL488-67075)

    IF Staining of Human liver cancer using CL488-67075

    Immunofluorescent analysis of (4% PFA) fixed Human liver cancer tissue using CoraLite® Plus 488 Endoglin/CD105 antibody (CL488-67075, Clone: 2D5E8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HUVEC cells using CoraLite® Plus 488-conjugated Endoglin/CD105 Monoc (CL488-67075)

    FC experiment of HUVEC using CL488-67075

    1X10^6 HUVEC cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Endoglin/CD105 (CL488-67075, Clone:2D5E8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFhumanes Leberkarzinomgewebe
Erfolgreiche Detektion in FCHUVEC-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67075 bindet in IF, FC (Intra) Endoglin/CD105 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Endoglin/CD105 fusion protein Ag27837
Vollständiger Name endoglin
Berechnetes Molekulargewicht 71 kDa
Beobachtetes Molekulargewicht 95-100 kDa
GenBank-ZugangsnummerBC014271
Gene symbol ENG
Gene ID (NCBI) 2022
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 Endoglin/CD105 antibody CL488-67075Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen