F4/80 Polyklonaler Antikörper

F4/80 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(307)

Rezensionen (1)

Kat-Nr. : 28463-1-AP

Synonyme

Adgre1, Adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, Emr1

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  WB analysis using 28463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28463-1-AP (F4/80 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 28463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 28463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 28463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse liver using 28463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 28463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 28463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of RAW 264.7 using 28463-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using F4/80 antibody (28463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: unboiled J774A.1 cells, unboiled RAW 264.7 cells
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Maushirngewebe, Mausherzgewebe, Mauslebergewebe, Mauslungengewebe, Maus-Dünndarmgewebe, Rattenlebergewebe, Rattenmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausmilzgewebe, Mauslebergewebe
• Erfolgreiche Detektion in IF-Fro: Mausmilzgewebe
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 20 publications below
• IHC: See 147 publications below
• IF: See 138 publications below

Produktinformation

28463-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA F4/80 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: F4/80 fusion protein Ag28252
• Vollständiger Name: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 130 kDa, 160 kDa
• GenBank-Zugangsnummer: NM_010130
• Gene symbol: F4/80
• Gene ID (NCBI): 13733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

Publikationen

Species	Application	Title
	IF	Bioact Mater; Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis; Authors - Wei Zhang
mouse	IF	Nat Commun; Dubosiella newyorkensis modulates immune tolerance in colitis via the L-lysine-activated AhR-IDO1-Kyn pathway; Authors - Yanan Zhang
human	IF	Nat Commun; Selectively targeting the AdipoR2-CaM-CaMKII-NOS3 axis by SCM-198 as a rapid-acting therapy for advanced acute liver failure; Authors - Rui Wang
rat,human	WB	Adv Sci (Weinh); Hierarchical Targeting Nanodrug with Holistic DNA Protection for Effective Treatment of Acute Kidney Injury; Authors - Qiaohui Chen
	IF	Adv Sci (Weinh); LIMA1 O-GlcNAcylation Promotes Hepatic Lipid Deposition through Inducing β-catenin-Regulated FASn Expression in Metabolic Dysfunction-Associated Steatotic Liver Disease; Authors - Fuji Yang
human	IF	Cell Rep Med; Lnc-H19-derived protein shapes the immunosuppressive microenvironment of glioblastoma; Authors - Junju Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kannan (Verified Customer) (06-13-2023)

AR using EDTApH9. overnight incubation of primary antibody at 4c.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse uterus