FADD Rekombinanter Antikörper

FADD Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241976A11

Kat-Nr. : 84619-5-RR

Synonyme

241976A11, FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, MORT1

---

• 

  WB analysis using 84619-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84619-5-RR (FADD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 84619-5-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84619-5-RR (FADD antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 84619-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FADD antibody (84619-5-RR, Clone: 241976A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of A431 using 84619-5-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug FADD Recombinant antibody (84619-5-RR, Clone:241976A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HT-1080 using 84619-5-RR

  1x10^6 HT-1080 cells were intracellularly stained with 0.25 ug FADD Recombinant antibody (84619-5-RR, Clone:241976A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 84619-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84619-5-RR against Human FADD were performed. The affinity constant is 19.8 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen, HT-1080.Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84619-5-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA FADD und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FADD fusion protein Ag6701
• Vollständiger Name: Fas (TNFRSF6)-associated via death domain
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC000334
• Gene symbol: FADD
• Gene ID (NCBI): 8772
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)