FAM13A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FAM13A Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 55401-1-AP

Synonyme

FAM13A, FAM13A1, KIAA0914, Protein FAM13A

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 55401-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55401-1-AP (FAM13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 55401-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 55401-1-AP (FAM13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 55401-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 55401-1-AP (FAM13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 55401-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 55401-1-AP (FAM13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsil using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsil using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsil using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsil using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55401-1-AP

  Immunofluorescent analysis of HeLa cells using 55401-1-AP (FAM13A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Maus-Eierstockgewebe, Mauslungengewebe, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Tonsillengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

55401-1-AP bindet in WB, IHC, IF, ELISA FAM13A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: family with sequence similarity 13, member A
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: NM_014883
• Gene symbol: FAM13A
• Gene ID (NCBI): 10144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM13A, also named as FAM13A1 and KIAA0914, belongs to the FAM13 family. It is associated with chronic obstructive pulmonary disease.(PMID: 20173748) This antibody is specific to FAM13A. It can recognize all the isoforms of FAM13A.

Publikationen

Species	Application	Title
mouse	WB	J Immunol; Annexin A2 Regulates Autophagy in Pseudomonas aeruginosa Infection through the Akt1-mTOR-ULK1/2 Signaling Pathway.; Authors - Rongpeng Li
	WB, IHC	Front Physiol; A Protective Role of FAM13A in Human Airway Epithelial Cells Upon Exposure to Cigarette Smoke Extract.; Authors - Qing Chen
human	WB	Mol Med Rep; miR‑30a‑5p induces the adipogenic differentiation of bone marrow mesenchymal stem cells by targeting FAM13A/Wnt/β‑catenin signaling in aplastic anemia.; Authors - Enbo Wang ; KD Validated
human	IHC	Am J Physiol Lung Cell Mol Physiol; FAM13A regulates cellular senescence marker p21 and mitochondrial reactive oxygen species production in airway epithelial cells; Authors - Qing Chen
human	IF	Am J Respir Cell Mol Biol; AKT Phosphorylates FAM13A and Promotes Its Degradation via CUL4A/DDB1/DCAF1 E3 Complex; Authors - Lu Gong