FAM164C Polyklonaler Antikörper

FAM164C Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20520-1-AP

Synonyme

Zinc finger C2HC domain-containing protein 1C, ZC2HC1C, C14orf140

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  WB analysis of HeLa using 20520-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20520-1-AP (FAM164C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20520-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20520-1-AP (FAM164C Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of C6 using 20520-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 20520-1-AP (FAM164C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 20520-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 20520-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 20520-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20520-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of C6 using 20520-1-AP

  Immunofluorescent analysis of C6 cells using 20520-1-AP (FAM164C antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: C6-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20520-1-AP bindet in WB, IHC, IF/ICC, ELISA FAM164C und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM164C fusion protein Ag14359
• Vollständiger Name: family with sequence similarity 164, member C
• Berechnetes Molekulargewicht: 275 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31-36 kDa
• GenBank-Zugangsnummer: BC004259
• Gene symbol: FAM164C
• Gene ID (NCBI): 79696
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM164C, also named as ZC2HC1C or C14orf140, is a 456 amino acid protein, which contains one C2HC-type zinc finger and belongs to the ZC2HC1 family. FAM164C exists as two isoforms. The isoform1 is a 51kDa protein ad isoform2 is 31kDa.