FAM3C Polyklonaler Antikörper

FAM3C Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 14247-1-AP

Synonyme

FAM3C, GS3786, ILEI, Interleukin like EMT inducer, Protein FAM3C

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Galerie der Validierungsdaten

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  WB analysis using 14247-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14247-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14247-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14247-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14247-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14247-1-AP (FAM3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14247-1-AP (FAM3C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HEK-293-Zellen, HepG2-Zellen, HT-29-Zellen, humanes Herzgewebe, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 3 publications below

Produktinformation

14247-1-AP bindet in WB, IHC, ELISA FAM3C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM3C fusion protein Ag5516
• Vollständiger Name: family with sequence similarity 3, member C
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC046932
• Gene symbol: FAM3C
• Gene ID (NCBI): 10447
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.

Publikationen

Species	Application	Title
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
human	WB	J Proteome Res; Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.; Authors - Loei Hendrick H
human	WB	Diagn Pathol; Prognostic significance of FAM3C in esophageal squamous cell carcinoma.; Authors - Ying-Hui Zhu
human	IHC	Int J Clin Exp Pathol; Overexpression of FAM3C protein as a novel biomarker for epithelial-mesenchymal transition and poor outcome in gastric cancer.; Authors - Shuai Yin
human,mouse	WB,IHC	Int J Biol Sci; A Novel ER Stress Mediator TMTC3 Promotes Squamous Cell Carcinoma Progression by Activating GRP78/PERK Signaling Pathway; Authors - Hongyu Yuan
human	IHC	Sci Rep; FAM3 family genes are associated with prognostic value of human cancer: a pan-cancer analysis; Authors - Qing-Tai Dong