FAM96B Polyklonaler Antikörper

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• KD/KO Validated

FAM96B Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 20108-1-AP

Synonyme

CGI 128, CIAB, CIAO2B, FAM96B, HSPC118, MIP18, Protein FAM96B

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  WB analysis using 20108-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20108-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 20108-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 20108-1-AP

  IP result of anti-FAM96B (IP:20108-1-AP, 4ug; Detection:20108-1-AP 1:300) with K-562 cells lysate 2400 ug.

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  IHC staining of mouse kidney using 20108-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 20108-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 20108-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20108-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, Ramos-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• CoIP: See 1 publications below

Produktinformation

20108-1-AP bindet in WB, IHC, IP, CoIP, ELISA FAM96B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM96B fusion protein Ag13827
• Vollständiger Name: family with sequence similarity 96, member B
• Berechnetes Molekulargewicht: 163 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC001733
• Gene symbol: FAM96B
• Gene ID (NCBI): 51647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM96B, also known as MIP18 or CIAO2B (Cytosolic iron-sulfur assembly component 2B), is a component of the cytosolic iron-sulfur protein assembly (CIA) complex involved in iron homeostasis and part of the MMXD complex contributed to proper chromosome segregation (PMID: 36503010). FAM96B may act as a scaffold protein to recruit CKB and the other components of the MMXD complex to associate with the spindle and mitotic apparatus and contributes to chromosome segregation.

Publikationen

Species	Application	Title
human	WB	Science; MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.; Authors - Oliver Stehling ; KD Validated
human	WB	Nat Struct Mol Biol; Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.; Authors - Nima N Naseri
human	WB	Cell Metab; Human CIA2A-FAM96A and CIA2B-FAM96B Integrate Iron Homeostasis and Maturation of Different Subsets of Cytosolic-Nuclear Iron-Sulfur Proteins.; Authors - Stehling Oliver O ; KD Validated
human	WB	Mol Cell; An Oxygen-Dependent Interaction between FBXL5 and the CIA-Targeting Complex Regulates Iron Homeostasis.; Authors - Adarsh K Mayank
human	WB	Cell Rep; The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.; Authors - Diana C Odermatt
human	WB	J Biol Chem; The Association of the DNA helicase XPD with Transcription Factor IIH is regulated by the Cytosolic Iron-sulfur Cluster Assembly Pathway.; Authors - Ajay A Vashisht