FBL Monoklonaler Antikörper

FBL Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

3A9E8

Kat-Nr. : 66985-1-Ig

Synonyme

FLRN, fibrillarin, FIB1, FIB, EC:2.1.1.-

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  WB analysis using 66985-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66985-1-Ig (FBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 66985-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66985-1-Ig (FBL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 66985-1-Ig

  IP result of anti-FBL (IP:66985-1-Ig, 5ug; Detection:66985-1-Ig 1:1000) with HeLa cells lysate 1600 ug.

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  IHC staining of human liver cancer using 66985-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66985-1-Ig (FBL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66985-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66985-1-Ig (FBL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66985-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

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  IF Staining of HeLa using 66985-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

• 

  IF Staining of HepG2 using 66985-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66985-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FBL (66985-1-Ig, Clone:3A9E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HeLa using 66985-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug FBL Monoclonal antibody (66985-1-Ig, Clone:3A9E8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 5 publications below
• RIP: See 1 publications below

Produktinformation

66985-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA FBL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FBL fusion protein Ag8841
• Vollständiger Name: fibrillarin
• Berechnetes Molekulargewicht: 321 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC019260
• Gene symbol: FBL
• Gene ID (NCBI): 2091
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).

Publikationen

Species	Application	Title
human	WB,IF	Gastroenterology; Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis; Authors - Xiao-Mei Yang
human	WB,RIP,IF	Mol Cell; lncRNAs maintain the functional phase state of nucleolar prion-like protein to facilitate rRNA processing; Authors - Yu-Meng Sun
human	WB	BMC Biol; A pair of long intergenic non-coding RNA LINC00887 variants act antagonistically to control Carbonic Anhydrase IX transcription upon hypoxia in tongue squamous carcinoma progression; Authors - Tao Shen
human	WB	Thorac Cancer; LncRNA FAM83A-AS1 promotes lung adenocarcinoma progression by enhancing the pre-mRNA stability of FAM83A.; Authors - Wenyi Wang
human	IF	Cell Death Discov; Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage; Authors - Jingtao Liu
human	WB,IF	Nat Commun; NAP-seq reveals multiple classes of structured noncoding RNAs with regulatory functions; Authors - Shurong Liu