Fibulin 5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Fibulin 5 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 12188-1-AP

Synonyme

ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, UP50, Urine p50 protein

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Galerie der Validierungsdaten

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  WB analysis using 12188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 12188-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12188-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 12188-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 12188-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 12188-1-AP

  IP Result of anti-Fibulin 5 (IP:12188-1-AP, 3ug; Detection:12188-1-AP 1:300) with mouse heart tissue lysate 8000ug.

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  IHC staining of mouse lung using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12188-1-AP (Fibulin 5 antibody at dilution of 1:200.

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  IHC staining of mouse lung using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12188-1-AP (Fibulin 5 antibody at dilution of 1:200.

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  IHC staining of human pancreas using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12188-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mausnierengewebe, Maus-Plazenta-Gewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe, Rattenhirngewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Mauslungengewebe, humanes Herzgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12188-1-AP bindet in WB, IP, IHC, IF, ELISA Fibulin 5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Fibulin 5 fusion protein Ag2859
• Vollständiger Name: fibulin 5
• Berechnetes Molekulargewicht: 448 aa, 50 kDa
• Beobachtetes Molekulargewicht: 55 kDa, 65 kDa
• GenBank-Zugangsnummer: BC022280
• Gene symbol: FBLN5
• Gene ID (NCBI): 10516
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50 kDa or 66 kDa band in western blotting, the 66 kDa corresponding to fully glycosylated protein.

Publikationen

Species	Application	Title
mouse	WB	Arterioscler Thromb Vasc Biol; Increased aortic stiffness and attenuated lysyl oxidase activity in obesity.; Authors - Chen Ju-Yi JY
human	WB	Biochem J; Fibulin-5 binds urokinase-type plasminogen activator and mediates urokinase-stimulated β1-integrin-dependent cell migration.; Authors - Kapustin Alexander A
mouse	WB, IHC	Cell Tissue Res; Aberrant elastin remodeling in the lungs of O2-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin.; Authors - Wenli Han
mouse	WB	Am J Physiol Cell Physiol; Caveolin-1 stabilizes ATP7A, Cu transporter for Extracellular SOD, in Vascular Tissue to Maintain Endothelial Function.; Authors - Varadarajan Sudhahar
human	WB	Clin Exp Pharmacol Physiol; Limb remote ischemic postconditioning-induced elevation of fibulin-5 confers neuroprotection to rats with cerebral ischemia/reperfusion injury: activation of the AKT pathway.; Authors - Wei Zhang ; KD Validated
human	IF	JID Innov; Fibulin-4 Accelerates Amyloid Formation by Binding with a Keratin 5 Peptide Fragment.; Authors - Fumihiko Katagiri