fibrillin 2-Specific Polyklonaler Antikörper

fibrillin 2-Specific Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20252-1-AP

Synonyme

CCA, DA9, FBN2, fibrillin 2, fibrillin 2-Specific

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Galerie der Validierungsdaten

• 

  WB analysis of human brain using 20252-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20252-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20252-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20252-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20252-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 20252-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20252-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 20252-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 20252-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 20252-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 20252-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HepG2-Zellen, L02-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

20252-1-AP bindet in WB, IHC, ELISA fibrillin 2-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: fibrillin 2
• Berechnetes Molekulargewicht: 315 kDa
• Beobachtetes Molekulargewicht: 290 kDa, 160 kDa
• GenBank-Zugangsnummer: NM_001999
• Gene symbol: FBN2
• Gene ID (NCBI): 2201
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBN2 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils which occur either in association with elastin or in elastin-free bundles. Fibrillin-2-containing microfibrils regulate the early process of elastic fiber assembly. Defects in FBN2 are the cause of congenital contractural arachnodactyly (CCA) which also known as Beals syndrome or distal arthrogryposis type 9 (DA9). This antibody is specific to FBN2. It recognizes both two isoforms ~300 kDa and 160 kDa.

Publikationen

Species	Application	Title
human	WB	Head Neck; High expression of NFX1-123 in HPV positive head and neck squamous cell carcinomas.; Authors - Sreenivasulu Chintala
mouse	IHC	J Ovarian Res; hUMSC transplantation restores follicle development in ovary damaged mice via re-establish extracellular matrix (ECM) components; Authors - Yin Shuyuan