FBXO28 Polyklonaler Antikörper

FBXO28 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24282-1-AP

Synonyme

F box only protein 28, F box protein 28, Fbx28, FBXO28, KIAA0483

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  WB analysis using 24282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24282-1-AP (FBXO28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 24282-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24282-1-AP (FBXO28 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 24282-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24282-1-AP (FBXO28 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 24282-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24282-1-AP (FBXO28 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 24282-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24282-1-AP (FBXO28 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

24282-1-AP bindet in WB, IHC, ELISA FBXO28 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBXO28 fusion protein Ag14900
• Vollständiger Name: F-box protein 28
• Berechnetes Molekulargewicht: 368 aa, 41 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC031576
• Gene symbol: FBXO28
• Gene ID (NCBI): 23219
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXO28 also named as F box only protein 28 is a 368 amino-acid protein, which contain 1 F-box domain and belongs to F-box protein family. FBXO28 is a critical component of SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. FBOX28 encodes a cell cycle regulated protein with a critical function in tumor cell proliferation. FBXO28 assembles an SCF-FBXO28 ubiquitin ligase whose activity is required during progression through the cell cycle by cyclin-dependent kinase 1/2 mediated phosphorylation. FBXO28 expression and phosphorylation to be strongly associated with poor prognosis and worse overall survival in human breast cancer.

Publikationen

Species	Application	Title
human	IHC	Oncogene; FBXO28 suppresses liver cancer invasion and metastasis by promoting PKA-dependent SNAI2 degradation; Authors - Xinran Qiao