Fc Epsilon RI Alpha Polyklonaler Antikörper

Fc Epsilon RI Alpha Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10980-1-AP

Synonyme

Fc epsilon RI alpha, FCE1A, FCER1A, FcERI, FceRIa, IgE Fc receptor subunit alpha

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Galerie der Validierungsdaten

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  WB analysis using 10980-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10980-1-AP (Fc Epsilon RI Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 10980-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 10980-1-AP (Fc epsilon RI alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 10980-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10980-1-AP (Fc epsilon RI alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MC/9-Zellen, humanes Milzgewebe, Mausmilzgewebe, Rattenmilzgewebe, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

10980-1-AP bindet in WB, IHC, IF, ELISA Fc Epsilon RI Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Fc Epsilon RI Alpha fusion protein Ag1398
• Vollständiger Name: Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 55-70 kDa
• GenBank-Zugangsnummer: BC015195
• Gene symbol: FCER1A
• Gene ID (NCBI): 2205
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide, also known as high affinity immunoglobulin epsilon receptor subunit alpha, FCER1A and FCE1A, is a single-pass type I membrane protein which contains 2 immunoglobulin-like domains. FCER1A is a subunit of the IgE receptor, which is composed of one glycosylated alpha (FCER1A), one beta (FCER1B), and two gamma (FCER1G) subunits. The high affinity IgE receptor plays a central role in allergic disease, coupling allergen and mast cells to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The calculated molecular weight of FCER1A is 30 kDa, the 55- to 70-kDa bands detected by this antibody are probably caused by heterogeneous glycosylation (PMID: 11344350; 12671054).

Publikationen

Species	Application	Title
mouse	IF	Science; Response to Comment on "Tumor-initiating cells establish an IL-33-TGF-β niche signaling loop to promote cancer progression".; Authors - Sachiko Taniguchi
human	IHC	J Allergy Clin Immunol; Mast cell-associated alveolar inflammation in patients with atopic uncontrolled asthma.; Authors - Andersson Cecilia K CK
human	WB	J Allergy Clin Immunol; Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces TH2/TH17 polarization through dectin-2.; Authors - Yunsheng Liang
mouse	WB	Transl Res; Allergic asthma aggravates angiotensin Ⅱ-induced cardiac remodeling in mice.; Authors - Chi Geng ; KO Validated
mouse	WB	Theranostics; Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p.; Authors - Hongmei Zhao
human	IHC	Thorax; Novel site-specific mast cell subpopulations in the human lung.; Authors - Andersson C K CK

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