FceR1a Polyklonaler Antikörper

FceR1a Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 10980-1-AP

Synonyme

IgE Fc receptor subunit alpha, FceRIa, FcERI, Fc-epsilon RI-alpha, FCE1A

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  WB analysis of human spleen using 10980-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 10980-1-AP (Fc epsilon RI alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10980-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10980-1-AP (Fc Epsilon RI Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 10980-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10980-1-AP (Fc epsilon RI alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MC/9-Zellen, humanes Milzgewebe, Mausmilzgewebe, Rattenmilzgewebe, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

10980-1-AP bindet in WB, IHC, IF, ELISA FceR1a und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FceR1a fusion protein Ag1398
• Vollständiger Name: Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 55-70 kDa
• GenBank-Zugangsnummer: BC015195
• Gene symbol: FceR1a
• Gene ID (NCBI): 2205
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex, FceRI, with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316). The calculated molecular weight of FceR1a is 30 kDa, the 55- to 70-kDa bands detected by this antibody are probably caused by heterogeneous glycosylation (PMID: 11344350; 12671054).

Publikationen

Species	Application	Title
mouse	IF	Science; Response to Comment on "Tumor-initiating cells establish an IL-33-TGF-β niche signaling loop to promote cancer progression".; Authors - Sachiko Taniguchi
human	IHC	J Allergy Clin Immunol; Mast cell-associated alveolar inflammation in patients with atopic uncontrolled asthma.; Authors - Andersson Cecilia K CK
human	WB	J Allergy Clin Immunol; Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces TH2/TH17 polarization through dectin-2.; Authors - Yunsheng Liang
mouse	WB	Transl Res; Allergic asthma aggravates angiotensin Ⅱ-induced cardiac remodeling in mice.; Authors - Chi Geng ; KO Validated
mouse	WB	Theranostics; Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p.; Authors - Hongmei Zhao
human	IHC	Thorax; Novel site-specific mast cell subpopulations in the human lung.; Authors - Andersson C K CK