FECH Polyklonaler Antikörper

FECH Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(24)

Rezensionen (2)

Kat-Nr. : 14466-1-AP

Synonyme

EC:4.98.1.1, EPP, EPP1, FCE, Protoheme ferro-lyase

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  WB analysis using 14466-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14466-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14466-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14466-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14466-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14466-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 14466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14466-1-AP (FECH Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 14466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14466-1-AP (FECH Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HepG2-Zellen, humanes Herzgewebe, humanes Lebergewebe, K-562-Zellen, Mausnierengewebe, Mauslebergewebe, Rattenherzgewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 23 publications below
• IHC: See 2 publications below

Produktinformation

14466-1-AP bindet in WB, IHC, ELISA FECH und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FECH fusion protein Ag5814
• Vollständiger Name: ferrochelatase (protoporphyria)
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 40-48 kDa
• GenBank-Zugangsnummer: BC039841
• Gene symbol: FECH
• Gene ID (NCBI): 2235
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FECH(ferrochelatase, mitochondrial ) is also named as heme synthase, protoheme ferro-lyase and belongs to the ferrochelatase family. It is a homodimeric (86 kDa) mitochondrial membrane-associated enzyme that catalyzes the insertion of ferrous iron into protoporphyrin to form heme(PMID:11175906). It has 2 isoforms produced by alternative splicing and the full length protein has a transit peptide with 54 amino acids.

Publikationen

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang
human	WB	Cancer Discov; NCOA4-Mediated Ferritinophagy Is a Pancreatic Cancer Dependency via Maintenance of Iron Bioavailability for Iron-Sulfur Cluster Proteins; Authors - Naiara Santana-Codina
human	WB	Cell Metab; A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.; Authors - Nunziata Maio
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
human	WB	J Am Coll Cardiol; Heme levels are increased in human failing hearts.; Authors - Khechaduri Arineh A
mouse	WB	Nat Commun; PMID: 32019928; Authors - Erika M Palmieri

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sofia (Verified Customer) (06-25-2025)

The antibody worked very well in western immunoblotting using 30µg liver lysates of murine origin.

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 800, 5% milk/TBST
• Cell Tissue Type: murine liver whole organ homogenates

FH

Marina (Verified Customer) (07-24-2024)

Incubation with primary antibody ON at 4C. Incubation with secondary antibody (rabbit) for 2h at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human cell lines