FER Monoklonaler Antikörper

FER Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1E3H2

Kat-Nr. : 67671-1-Ig

Synonyme

FER, p94 Fer, Proto oncogene c Fer, TYK3, Tyrosine kinase 3, Tyrosine protein kinase Fer

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  WB analysis using 67671-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67671-1-Ig (FER antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of ROS1728 using 67671-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 67671-1-Ig (FER antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67671-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67671-1-Ig (FER antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 67671-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using FER antibody (67671-1-Ig, Clone: 1E3H2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67671-1-Ig bindet in WB, IF/ICC, ELISA FER und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FER fusion protein Ag19176
• Vollständiger Name: fer (fps/fes related) tyrosine kinase
• Berechnetes Molekulargewicht: 822 aa, 95 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC017060
• Gene symbol: FER
• Gene ID (NCBI): 2241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.

Publikationen

Species	Application	Title
human	WB	Cell Rep; THBS2-producing matrix CAFs promote colorectal cancer progression and link to poor prognosis via the CD47-MAPK axis; Authors - Zaoqu Liu