Kindlin 1 Polyklonaler Antikörper

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• KD/KO Validated

Kindlin 1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 22215-1-AP

Synonyme

C20orf42, DTGCU2, Fermitin family homolog 1, FERMT1, KIND1, Kindlerin, Kindlin 1, Kindlin syndrome protein, Unc 112 related protein 1, UNC112A, URP1

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 22215-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22215-1-AP (FERMT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 22215-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22215-1-AP (FERMT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22215-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22215-1-AP (FERMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (FERMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (FERMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (FERMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 22215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (FERMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 22215-1-AP

  Immunofluorescent analysis of HEK-293 cells using 22215-1-AP (FERMT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, COLO 320-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Kolonkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

22215-1-AP bindet in WB, IHC, IF, ELISA Kindlin 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Kindlin 1 fusion protein Ag17543
• Vollständiger Name: fermitin family homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 677 aa, 77 kDa
• Beobachtetes Molekulargewicht: 70-77 kDa
• GenBank-Zugangsnummer: BC035882
• Gene symbol: FERMT1
• Gene ID (NCBI): 55612
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kindlin-1 is a FERM domain containing adaptor protein that is found predominantly at cell-extracellular matrix adhesions where it binds to β-integrin subunits and is required for integrin activation. Loss of function mutations in the FERMT1 gene which encodes Kindlin-1 leads to the development of Kindler Syndrome (KS) an autosomal recessive skin disorder characterized by skin blistering, photosensitivity, and predisposition to aggressive squamous cell carcinoma (SCC).

Publikationen

Species	Application	Title
human	WB,IHC	Cancer Cell Int; FERMT1 contributes to the migration and invasion of nasopharyngeal carcinoma through epithelial-mesenchymal transition and cell cycle arrest.; Authors - Lingling Li ; KD Validated
rat	WB	Eur J Neurosci; Wnt10a/β-catenin signaling is involved in kindlin-1-mediated astrocyte activation in a chronic construction injury rat model.; Authors - Baisong Zhao
mouse	IHC,IF	Int J Mol Med; Kindlin-1 contributes to EGF-induced re-epithelialization in skin wound healing.; Authors - Congcong Shen
mouse,rat	WB,IF	Exp Anim; FERMT1 knockdown attenuates inflammation induced by intracerebral hemorrhage in rats via NLRP3/NF-κB pathway; Authors - Jianqiang Wei ; KD Validated
mouse	WB	Cell Rep; Distinct bidirectional regulation of LFA1 and α4β7 by Rap1 and integrin adaptors in T cells under shear flow; Authors - Yuji Kamioka