FGD2 Polyklonaler Antikörper

FGD2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27068-1-AP

Synonyme

Zinc finger FYVE domain-containing protein 4, ZFYVE4, FYVE, RhoGEF and PH domain-containing protein 2

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  WB analysis of Raji using 27068-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27068-1-AP (FGD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27068-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27068-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27068-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 27068-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27068-1-AP bindet in WB, IHC, ELISA FGD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FGD2 fusion protein Ag25782
• Vollständiger Name: FYVE, RhoGEF and PH domain containing 2
• Berechnetes Molekulargewicht: 655 aa, 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC023645
• Gene symbol: FGD2
• Gene ID (NCBI): 221472
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FGD2 is a member of the formin family of proteins, which are known for their role in regulating the assembly of actin filaments. Formins are characterized by their ability to nucleate and elongate actin filaments, and they play a crucial role in processes that require dynamic actin structures, such as cell motility and cell division. Mutations or alterations in FGD2 have been associated with certain developmental disorders and diseases. For example, mutations in FGD2 are a cause of faciogenital dysplasia (Aarskog-Scott syndrome), a condition characterized by facial and genital abnormalities and short stature.