FGFR1 Monoklonaler Antikörper

FGFR1 Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2c

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

26

CloneNo.

10F12E7

Kat-Nr. : 60325-1-Ig

Synonyme

bFGF R 1, BFGFR, CD331, CEK, FGFBR, FGFR 1, FGFR1, FLG, FLT 2, FLT2, Fms like tyrosine kinase 2, HBGFR, KAL2, N SAM, OGD, Proto oncogene c Fgr

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 60325-1-Ig

  MCF-7, HT-1080, HUVEC, U2OS, Y79, and K-562 cells were subjected to SDS PAGE followed by western blot with 60325-1-Ig (FGFR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 60325-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60325-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of K-562 using 60325-1-Ig

  1X10^6 K-562 cells were stained with 0.2ug FGFR1 antibody (60325-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HT-1080.Zellen, HUVEC-Zellen, K-562-Zellen, U2OS-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 23 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Produktinformation

60325-1-Ig bindet in WB, IHC, IF, FC, ELISA FGFR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2c
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FGFR1 fusion protein Ag3646
• Vollständiger Name: fibroblast growth factor receptor 1
• Berechnetes Molekulargewicht: 820 aa, 91 kDa
• Beobachtetes Molekulargewicht: 91 kDa
• GenBank-Zugangsnummer: BC018128
• Gene symbol: FGFR1
• Gene ID (NCBI): 2260
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processes including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in the FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer, and autosomal dominant Kallmann syndrome. Alternatively spliced variants that encode different protein isoforms have been described.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
human	WB	Theranostics; Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis.; Authors - Meng Wang
human	WB,IHC	J Transl Med; A novel intronic circular RNA circFGFR1int2 up-regulates FGFR1 by recruiting transcriptional activators P65/FUS and suppressing miR-4687-5p to promote prostate cancer progression; Authors - Ruyue Wang
human	WB	Oxid Med Cell Longev; DJ-1/FGFR-1 Signaling Pathway Contributes to Sorafenib Resistance in Hepatocellular Carcinoma.; Authors - Xin Chen
human	WB	Int J Mol Sci; The FGFR Family Inhibitor AZD4547 Exerts an Antitumor Effect in Ovarian Cancer Cells.; Authors - Yu Ran Na
	WB	J Cell Physiol; Brivanib, a multitargeted small-molecule tyrosine kinase inhibitor, suppresses laser-induced CNV in a mouse model of neovascular AMD.; Authors - Lele Li

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