FGFR1 Monoklonaler Antikörper

FGFR1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2c

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(41)

CloneNo.

10F12E7

Rezensionen (1)

Kat-Nr. : 60325-1-Ig

Synonyme

EC:2.7.10.1, CEK, CD331, bFGF-R-1, BFGFR

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• 

  WB analysis of MCF-7 using 60325-1-Ig

  MCF-7, HT-1080, HUVEC, U2OS, Y79, and K-562 cells were subjected to SDS PAGE followed by western blot with 60325-1-Ig (FGFR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 60325-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60325-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of ACHN using 60325-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed ACHN cells using FGFR1 antibody (60325-1-Ig, Clone: 10F12E7 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  FC experiment of K-562 using 60325-1-Ig

  1x10^6 K-562 cells were intracellularly stained with 0.2 ug FGFR1 Monoclonal antibody (60325-1-Ig, Clone:10F12E7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HT-1080.Zellen, HUVEC-Zellen, K-562-Zellen, U2OS-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: ACHN cells
• Erfolgreiche Detektion in FC (Intra): K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 37 publications below
• IHC: See 11 publications below
• IF: See 2 publications below

Produktinformation

60325-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA FGFR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2c
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FGFR1 fusion protein Ag3646
• Vollständiger Name: fibroblast growth factor receptor 1
• Berechnetes Molekulargewicht: 820 aa, 91 kDa
• Beobachtetes Molekulargewicht: 91 kDa
• GenBank-Zugangsnummer: BC018128
• Gene symbol: FGFR1
• Gene ID (NCBI): 2260
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processes including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in the FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer, and autosomal dominant Kallmann syndrome. Alternatively spliced variants that encode different protein isoforms have been described.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
human	WB	Int J Biol Sci; PROTAC-Surufatinib Suppresses Pancreatic Neuroendocrine Neoplasms Progression by Inducing Ferroptosis through Inhibiting WNT/β-catenin Pathway Mediated by HMOX1; Authors - Bingyan Xue
	IHC	ACS Appl Mater Interfaces; Hydrogel Loaded with Peptide-Containing Nanocomplexes: Symphonic Cooperation of Photothermal Antimicrobial Nanoparticles and Prohealing Peptides for the Treatment of Infected Wounds; Authors - Qiuye Jia
human	WB	Theranostics; Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis.; Authors - Meng Wang
human	WB,IHC	J Transl Med; A novel intronic circular RNA circFGFR1int2 up-regulates FGFR1 by recruiting transcriptional activators P65/FUS and suppressing miR-4687-5p to promote prostate cancer progression; Authors - Ruyue Wang
human	WB	Oxid Med Cell Longev; DJ-1/FGFR-1 Signaling Pathway Contributes to Sorafenib Resistance in Hepatocellular Carcinoma.; Authors - Xin Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (09-30-2025)

FGFR1 (in green) did not seem to work on IF on human brain epileptic FFPE tissue, appearing as a halo around the nuclei instead of on the membrane.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

FGFR1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:60325-1-Ig)