FH Polyklonaler Antikörper

FH Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10966-1-AP

Synonyme

FH, Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10966-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10966-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10966-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10966-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10966-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10966-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10966-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10966-1-AP

  IP Result of anti-FH (IP:10966-1-AP, 4ug; Detection:10966-1-AP 1:1000) with HepG2 cells lysate 2400ug.

• 

  IHC staining of human prostate cancer using 10966-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10966-1-AP (FH antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 10966-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10966-1-AP (FH Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human liver using 10966-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10966-1-AP (FH Antibody) at dilution of 1:50(under 40x lens).

• 

  IF Staining of HepG2 using 10966-1-AP

  Immunofluorescent analysis of HepG2 cells, using FH antibody 10966-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HepG2 using 10966-1-AP

  Immunofluorescent analysis of HepG2 cells using 10966-1-AP (FH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10966-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug FH antibody (10966-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Lebergewebe, L02-Zellen, Mauslebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10966-1-AP bindet in WB, IP, IHC, IF, FC, ELISA FH und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FH fusion protein Ag1437
• Vollständiger Name: fumarate hydratase
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC017444
• Gene symbol: FH
• Gene ID (NCBI): 2271
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts a tumor suppressor functions of FH in sporadic kidney cancer(PMID:21695080). This protein has 2 isoforms produced by alternative initiation.

Publikationen

Species	Application	Title
human	WB	Oncotarget; Identification of SPOP related metabolic pathways in prostate cancer.; Authors - Min Yan
human	IF	Endocr Relat Cancer; Cellular kinetics of MED12-mutant uterine leiomyoma growth and regression in vivo.; Authors - Vanida Ann Serna
human,mouse	WB,IHC	Pharmaceuticals (Basel); Identification of a Difluorinated Alkoxy Sulfonyl Chloride as a Novel Antitumor Agent for Hepatocellular Carcinoma through Activating Fumarate Hydratase Activity; Authors - Jin Jin