FHL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FHL1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch

Anwendung

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(32)

Kat-Nr. : 10991-1-AP

Synonyme

FHL 1, FHL-1, FHL1B, FLH1A, four and a half LIM domains 1

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  WB analysis using 10991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10991-1-AP

  WB result of FHL1 antibody (10991-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and sg-FHL1 transfected HEK-293 cells.

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  WB analysis of mouse colon using 10991-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10991-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10991-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10991-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10991-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 10991-1-AP

  IP result of anti-FHL1 (IP:10991-1-AP, 4ug; Detection:10991-1-AP 1:8000) with mouse skeletal muscle tissue lysate 4000 ug.

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  IHC staining of human ovary tumor using 10991-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10991-1-AP (FHL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 10991-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10991-1-AP (FHL1 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, HEK-293-Zellen, humanes Hirngewebe, Maus-Kolongewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 10 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

10991-1-AP bindet in WB, IHC, IF, IP, CoIP, ChIP, ELISA FHL1 und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FHL1 fusion protein Ag1448
• Vollständiger Name: four and a half LIM domains 1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC010998
• Gene symbol: FHL1
• Gene ID (NCBI): 2273
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Four-and-a-half LIM domains 1 (FHL1), a member of the FHL protein family, which composed of FHL 1, 2, 3, 4 and ACT (activator of CREM), and characterized by an N-terminal half LIM domain followed by four complete LIM domains. FHL1 may contribute to sarcomere synthesis, assembly and biomechanical stress sensing. FHL1 is also a methylation-silenced tumor-suppressor gene on chromosome X in gastrointestinal cancers, which contributes to the formation of an epigenetic field for cancerization. FHL1(10991-1-AP) is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. This is a rabbit polyantibody raised against full length of human FHL1.

Publikationen

Species	Application	Title
human	WB,IHC,CoIP,ChIP	J Clin Invest; Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.; Authors - Ding Lihua L ; KD Validated
human	WB	Genes Dev; Browning of human adipocytes requires KLF11 and reprogramming of PPARγ superenhancers.; Authors - Anne Loft ; KD Validated
human	WB,IHC	Nat Commun; A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.; Authors - Xiaojie Xu ; KD Validated
		J Cell Biol; Src-mediated phosphorylation converts FHL1 from tumor suppressor to tumor promoter.; Authors - Xiang Wang
human	WB,IHC	Int J Cancer; Downregulation and growth inhibitory role of FHL1 in lung cancer.; Authors - Niu Chang C
mouse,human	WB	J Cell Sci; MAL/MRTF-A controls migration of non-invasive cells by upregulation of cytoskeleton-associated proteins.; Authors - Leitner Laura L