FITC Rekombinanter Antikörper

FITC Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

FITC conjugated und mehr (1)

Anwendung

WB, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1B2

Kat-Nr. : 80003-1-RR

Synonyme

5-Isothiocyanat Fluorescein, Fluorescein

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  WB analysis using 80003-1-RR

  FITC-conjugated secondary antibodies were subjected to SDS PAGE followed by western blot with 80003-1-RR (FITC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: FITC conjugated antbody

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

80003-1-RR bindet in WB, IF, IP, ELISA FITC und zeigt Reaktivität mit FITC conjugated

• Getestete Reaktivität: FITC conjugated
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: FITC
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This rabbit recombinant antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.

Publikationen

Species	Application	Title
	IF	Drug Dev Res; Carnosol alleviates sevoflurane-induced cognitive dysfunction by mediating NF-κB pathway in aged rats; Authors - Qing Li
human	IP	Biomed Pharmacother; Fucoidan inhibits EGFR redistribution and potentiates sorafenib to overcome sorafenib-resistant hepatocellular carcinoma; Authors - Jialiang Luo